PXD025628

PXD025628 is an original dataset announced via ProteomeXchange.

Title	Protein landscape in acute myeloid leukemia reveals proteins susceptible for drug discovery
Description	Acute myeloid leukemia (AML) is a devastating cancer affecting the hematopoietic system. Although this disease represents only 35% of diagnosed leukemias, it accounts for nearly 50% of leukemia-related deaths, making it the leading cause of leukemia-related mortality. Although many AML initiating mutations have been identified, the downstream effects leading to disease progression are still largely unknown. Previous research has relied on RNA sequencing and microarray techniques to study the downstream effects, providing data at the transcriptional level. While these studies have proven efficacious, they fail to capture the changes that occur at the proteomic level. To interrogate the effect of protein expression alterations in AML, we performed a quantitative mass spectrometry analysis using mouse models to compare three tumor types to untransformed tumor-initiating population. In parallel, we performed RNA sequencing for the same populations. With these combined results, we identified 34 proteins whose expression was upregulated in AML tumors, but strikingly, were unaltered at the transcriptional level. These proteins are shown to be associated with mitochondrial function as well as RNA processing. These studies identify a set of proteins not previously associated with leukemia, and may ultimately serve as potential targets for therapeutic manipulation.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-19_23:10:50.778.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samantha Swenson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-26 00:51:30	ID requested
⏵ 1	2022-05-19 23:10:51	announced

Caplan M, Wittorf KJ, Weber KK, Swenson SA, Gilbreath TJ, Willow Hynes-Smith R, Amador C, Hyde RK, Buckley SM, Multi-omics reveals mitochondrial metabolism proteins susceptible for drug discovery in AML. Leukemia, 36(5):1296-1305(2022) [pubmed]

submitter keyword: Acute myeloid leukemia, AML,

Shannon Mychel Buckley
contact affiliation	Department of Genetics, Cell Bioliogy, & Anatomy Buckley Lab University of Nebraska Medical Center Omaha, Nebraska United States of America
contact email	shannon.buckley@unmc.edu
lab head
Samantha Swenson
contact affiliation	University of Nebraska Medical Center
contact email	samantha.swenson@unmc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025628
     1. Label: PRIDE project
     2. Name: Protein landscape in acute myeloid leukemia reveals proteins susceptible for drug discovery