PXD025626

PXD025626 is an original dataset announced via ProteomeXchange.

Title	Critical functions of FAM134 family members in endoplasmic reticulum remodeling, ER-phagy and collagen quality control
Description	Degradation of the endoplasmic reticulum (ER) by selective autophagy (ER-phagy) is vital for cellular homeostasis. Here, we introduce FAM134A/RETREG2 and FAM134C/RETREG3 as new ER-phagy receptors. FAM134A and FAM134C exist in a relatively inactive state under basal conditions and require an activation signal to induce significant ER fragmentation. Molecular modeling and simulations implicate a single compact fold of FAM134A’s reticulon homology domain (RHD). In contrast, the RHDs of FAM134B and FAM134C are able to adopt at least three discrete conformations. These differences result in slower vesicle formation for FAM134A compared to FAM134C and mostly FAM134B. Global proteomic analyses of knockout MEFs indicate both distinct and overlapping roles for the Fam134s, with Fam134a being most distant from Fam134b and Fam134c. Fam134c appears to enhance and facilitate Fam134b’s role in maintaining pro-collagen-I levels and is therefore insufficient to compensate for its loss. By contrast, Fam134a has a particular mode of action in maintaining pro-collagen-I levels and is able to fully compensate loss of Fam134b or Fam134c upon over-expression in its wild-type or LIR mutant form.
HostingRepository	PRIDE
AnnounceDate	2021-09-14
AnnouncementXML	Submission_2021-09-14_08:17:41.573.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	carmine cirillo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-25 22:26:13	ID requested
⏵ 1	2021-09-14 08:17:41	announced

Dataset with its publication pending

submitter keyword: Autophagy, Collagen, ER-phagy, Endoplasmic Reticulum, FAM134, RETREG, U2OS.

Paolo Grumati
contact affiliation	Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy.
contact email	p.grumati@tigem.it
lab head
carmine cirillo
contact affiliation	TIGEM
contact email	c.cirillo@tigem.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025626

PRIDE project URI

• PRIDE
  1. PXD025626
     1. Label: PRIDE project
     2. Name: Critical functions of FAM134 family members in endoplasmic reticulum remodeling, ER-phagy and collagen quality control