PXD025592

PXD025592 is an original dataset announced via ProteomeXchange.

Title	Integrated physiological and comparative proteomics analysis of contrasting genotypes of pearl millet reveals underlying salt-responsive mechanisms
Description	Salinity stress poses a significant risk to agricultural yield and productivity. Therefore, elucidation of plant salt-response mechanisms has become essential to identify stress-tolerance genes. In the present study, two pearl millet genotypes with contrasting salt-tolerance showed differential morpho-physiological and proteomic responses under 150 mM NaCl, and the genotype IC 325825 could withstand salt-stress better than IP 17224. The salt-tolerance potential of IC 325825 was associated with its ability to maintain ionic, osmotic balance and membrane integrity under stress. The IC 325825 exhibited better growth under salinity as compared to IP 17224 due to higher expression of C4 photosynthesis enzymes, efficient antioxidant system, and lower Na+/K+ ratio. Comparative proteomics analysis revealed greater metabolic perturbation in IP 17224 under salinity, in contrast to IC 325825 that harbored pro-active stress-responsive machinery, allowing its survival and better adaptability under salt-stress. The differentially expressed proteins were in-silico characterized for their functions, subcellular-localization, pathway/ interaction analysis, and relative transcript levels. This study has provided novel insights into salinity stress adaptive mechanisms in pearl millet, demonstrating the power of proteomics-based approaches. The critical proteins identified in the present study could be further explored as potential objects for increasing salt-tolerance in sensitive crop plants.
HostingRepository	PRIDE
AnnounceDate	2022-05-09
AnnouncementXML	Submission_2022-05-16_05:43:28.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shweta Jha
SpeciesList	scientific name: Cenchrus americanus; NCBI TaxID: 4543;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-23 06:20:40	ID requested
1	2022-05-09 03:52:51	announced
⏵ 2	2022-05-16 05:43:29	announced	2022-05-16: Updated publication reference for PubMed record(s): 34837239.

Jha S, Maity S, Singh J, Chouhan C, Tak N, Ambatipudi K, Integrated physiological and comparative proteomics analysis of contrasting genotypes of pearl millet reveals underlying salt-responsive mechanisms. Physiol Plant, 174(1):e13605(2022) [pubmed]

submitter keyword: comparative proteomics

contrasting genotype

differential protein expression

pearl millet

salinity tolerance

stress signaling

Shweta Jha
contact affiliation	Plant Functional Genomics Lab, Biotechnology Unit, Department of Botany (UGC-Centre of Advance Study), Jai Narain Vyas University, Jodhpur, 342001, India
contact email	jha.shweta80@gmail.com
lab head
Shweta Jha
contact affiliation	Department of Botany (UGC- Centre of Advance Study), J.N.V. University-New Campus, Jodhpur (Raj.)- 342001
contact email	Jha.shweta80@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD025592

PRIDE project URI

• PRIDE
  1. PXD025592
     1. Label: PRIDE project
     2. Name: Integrated physiological and comparative proteomics analysis of contrasting genotypes of pearl millet reveals underlying salt-responsive mechanisms