PXD025585

PXD025585 is an original dataset announced via ProteomeXchange.

Title	Identification and relative quantification of hFSH glycoforms in women sera by PRM MS based approach.
Description	This study presents an alternative preliminary strategy to identify and quantify serum hFSH glycoforms based on immunopurification assay and mass spectrometry (MS) using parallel reaction monitoring (PRM) analysis. In this study, we provide MS-PRM data acquisition method for hFSH glycopeptides identification with high specificity and their quantification by extracting the chromatographic traces of selected fragments of glycopeptides. Once set up in all its features, the proposed method could be transferred to the clinic to improve the fertility treatments and follow-ups in men and women.
HostingRepository	PRIDE
AnnounceDate	2021-06-15
AnnouncementXML	Submission_2021-06-14_22:13:12.158.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara Melchiorre
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-23 06:20:06	ID requested
⏵ 1	2021-06-14 22:13:12	announced

Melchiorre C, Chhuon C, Jung V, Lipecka J, Di Rella F, Conforti A, Amoresano A, Carpentieri A, Guerrera IC, Identification and Relative Quantification of hFSH Glycoforms in Women's Sera via MS-PRM-Based Approach. Pharmaceutics, 13(6):(2021) [pubmed]

submitter keyword: PRM-Mass Spectrometry

FSH

Glycosylation

Ida Chiara Guerrera
contact affiliation	Proteomics platform Necker, Université de Paris –Structure Fédérative de Recherche Necker, INSERM US24/CNRS UMS3633, Paris 75015, France.
contact email	chiara.guerrera@inserm.fr
lab head
Chiara Melchiorre
contact affiliation	University of Naples Federico II
contact email	chiara.melchiorre@unina.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025585

PRIDE project URI

• PRIDE
  1. PXD025585
     1. Label: PRIDE project
     2. Name: Identification and relative quantification of hFSH glycoforms in women sera by PRM MS based approach.