PXD025576

PXD025576 is an original dataset announced via ProteomeXchange.

Title	Characterizing the role of OCIAD1 in the proteolytic processing of holocytochrome c1 and CIII2 assembly
Description	To characterize the nature of the cytochrome c1 (CYC1) processivity defect in native CIII2 assemblies we identified CYC1 peptides using mass spectrometry analysis of blue native (BN)-PAGE. Gel slices ranging from ~600-900kDa and containing CIII2 assemblies were excised from U2OS control cells, U2OS OCIAD1 knockdown cells, and U2OS OCIAD1 knockdown cells rescued with wildtype OCIAD1. Gel slices were then digested and analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2021-05-18
AnnouncementXML	Submission_2021-05-18_01:27:36.830.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maxence Le Vasseur
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-22 23:21:21	ID requested
⏵ 1	2021-05-18 01:27:37	announced

Dataset with its publication pending

submitter keyword: OCIAD1, mitochondria, Complex III, cytochrome c1, IMMP2L, processivity, cleavage

Jodi Nunnari
contact affiliation	Department of Molecular and Cellular Biology, College of Biological Sciences, University of California, Davis, CA, 95616, USA
contact email	jmnunnari@ucdavis.edu
lab head
Maxence Le Vasseur
contact affiliation	University of California, Davis
contact email	mlevasseur@ucdavis.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025576
     1. Label: PRIDE project
     2. Name: Characterizing the role of OCIAD1 in the proteolytic processing of holocytochrome c1 and CIII2 assembly