PXD025571 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteometabolomics for unsupervised identification of protein–metabolite interactions |
Description | Protein–metabolite interactions play an important role in the cell’s metabolism and many methods have been developed to screen them in vitro. However, few methods can be applied at a large scale and not alter biological state. Here we describe a proteometabolomic approach, using chromatography to generate cell fractions which are then analyzed with mass spectrometry for both protein and metabolite identification. Integrating the proteomic and metabolomic analyses makes it possible to identify protein-bound metabolites. Applying the concept to the thermophilic fungus Chaetomium thermophilum, we predict many likely protein-metabolite interactions, most of them novel. As a proof of principle, we experimentally validate a predicted interaction between the ribosome and isopentenyl adenine. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-23 |
AnnouncementXML | Submission_2021-04-22_23:52:25.754.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yuanyue Li |
SpeciesList | scientific name: Chaetomium thermophilum var. thermophilum DSM 1495; NCBI TaxID: 759272; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-22 23:15:04 | ID requested | |
⏵ 1 | 2021-04-22 23:52:26 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: protein–metabolite interactions, LC-MS/MS |
Contact List
Peer Bork |
contact affiliation | EMBL |
contact email | bork@embl.de |
lab head | |
Yuanyue Li |
contact affiliation | EMBL |
contact email | liyuanyue@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025571
- Label: PRIDE project
- Name: Proteometabolomics for unsupervised identification of protein–metabolite interactions