PXD025571

PXD025571 is an original dataset announced via ProteomeXchange.

Title	Proteometabolomics for unsupervised identification of protein–metabolite interactions
Description	Protein–metabolite interactions play an important role in the cell’s metabolism and many methods have been developed to screen them in vitro. However, few methods can be applied at a large scale and not alter biological state. Here we describe a proteometabolomic approach, using chromatography to generate cell fractions which are then analyzed with mass spectrometry for both protein and metabolite identification. Integrating the proteomic and metabolomic analyses makes it possible to identify protein-bound metabolites. Applying the concept to the thermophilic fungus Chaetomium thermophilum, we predict many likely protein-metabolite interactions, most of them novel. As a proof of principle, we experimentally validate a predicted interaction between the ribosome and isopentenyl adenine.
HostingRepository	PRIDE
AnnounceDate	2021-04-23
AnnouncementXML	Submission_2021-04-22_23:52:25.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuanyue Li
SpeciesList	scientific name: Chaetomium thermophilum var. thermophilum DSM 1495; NCBI TaxID: 759272;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-22 23:15:04	ID requested
⏵ 1	2021-04-22 23:52:26	announced

Dataset with its publication pending

submitter keyword: protein–metabolite interactions, LC-MS/MS

Peer Bork
contact affiliation	EMBL
contact email	bork@embl.de
lab head
Yuanyue Li
contact affiliation	EMBL
contact email	liyuanyue@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD025571

PRIDE project URI

• PRIDE
  1. PXD025571
     1. Label: PRIDE project
     2. Name: Proteometabolomics for unsupervised identification of protein–metabolite interactions