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PXD025571

PXD025571 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteometabolomics for unsupervised identification of protein–metabolite interactions
DescriptionProtein–metabolite interactions play an important role in the cell’s metabolism and many methods have been developed to screen them in vitro. However, few methods can be applied at a large scale and not alter biological state. Here we describe a proteometabolomic approach, using chromatography to generate cell fractions which are then analyzed with mass spectrometry for both protein and metabolite identification. Integrating the proteomic and metabolomic analyses makes it possible to identify protein-bound metabolites. Applying the concept to the thermophilic fungus Chaetomium thermophilum, we predict many likely protein-metabolite interactions, most of them novel. As a proof of principle, we experimentally validate a predicted interaction between the ribosome and isopentenyl adenine.
HostingRepositoryPRIDE
AnnounceDate2021-04-23
AnnouncementXMLSubmission_2021-04-22_23:52:25.754.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuanyue Li
SpeciesList scientific name: Chaetomium thermophilum var. thermophilum DSM 1495; NCBI TaxID: 759272;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-22 23:15:04ID requested
12021-04-22 23:52:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: protein–metabolite interactions, LC-MS/MS
Contact List
Peer Bork
contact affiliationEMBL
contact emailbork@embl.de
lab head
Yuanyue Li
contact affiliationEMBL
contact emailliyuanyue@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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