PXD025569

PXD025569 is an original dataset announced via ProteomeXchange.

Title	Unbiased quantitative analysis of the cardiac phosphoproteome in response to acute β-adrenergic receptor stimulation in vivo
Description	We utilised Super-SILAC mouse technology to perform an unbiased, quantitative analysis of the cardiac phosphoproteome in mice acutely treated in vivo with either saline or isoprenaline (ISO), a non-selective β-AR agonist. Using a customized mass spectrometry-based workflow (Figure 1), our study identified hundreds of phosphosites that are significantly regulated by β-AR stimulation, including both novel phosphoproteins and phosphosites that have not been previously identified as downstream effectors of β-AR stimulation and established downstream components of cAMP/PKA and CaMKII signaling pathways. Interestingly, our study revealed both up- and down-phosphorylation in comparable numbers, indicative of an intricate signaling network activated in response to β-AR stimulation.
HostingRepository	PRIDE
AnnounceDate	2021-12-30
AnnouncementXML	Submission_2021-12-30_14:51:45.967.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-22 23:09:14	ID requested
⏵ 1	2021-12-30 14:51:46	announced

G, ü, ran A, Ji Y, Fang P, Pan KT, Urlaub H, Avkiran M, Lenz C, -Adrenergic Receptor Stimulation In Vivo. Int J Mol Sci, 22(22):(2021) [pubmed]

submitter keyword: phosphoproteome

mass spectrometry

β-adrenergic receptor

SILAC

Christof Lenz
contact affiliation	Institute of Clinical Chemistry, University Medical Center Göttingen
contact email	christof.lenz@mpibpc.mpg.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD025569

PRIDE project URI

• PRIDE
  1. PXD025569
     1. Label: PRIDE project
     2. Name: Unbiased quantitative analysis of the cardiac phosphoproteome in response to acute β-adrenergic receptor stimulation in vivo