PXD025568

PXD025568 is an original dataset announced via ProteomeXchange.

Title	A single immunization with CAF08 provides newborns with Th1-mediated protection against Respiratory Syncytial Virus infection
Description	Respiratory Syncytial Virus (RSV) is a leading cause of morbidity and mortality in children, due in part to their distinct immune system, characterized by impaired induction of T-helper 1 (Th1)‐immunity. Here we describe cationic adjuvant formulation (CAF)-08, a liposomal vaccine formulation tailored to induce Th1 immunity in early life via synergistic engagement of Toll-like Receptor 7/8 and the C-type lectin receptor Mincle. Quantitative phosphoproteomics applied to human dendritic cells revealed a key role for Protein Kinase C- for enhanced Th1 cytokine production in neonatal dendritic cells and identified signaling events resulting in antigen cross-presentation. In vivo, a single immunization at birth with CAF08-adjuvanted RSV pre-fusion antigen protected newborn mice from RSV infection through induction of antigen-specific CD8+ and Th1 cells. Overall, we describe a novel pediatric adjuvant formulation and characterize its mechanism of action providing a promising avenue for development of early life vaccines against RSV and other intracellular pathogens.
HostingRepository	PRIDE
AnnounceDate	2022-06-14
AnnouncementXML	Submission_2022-06-14_13:37:40.890.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simon van Haren
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-22 23:08:03	ID requested
⏵ 1	2022-06-14 13:37:41	announced

Dataset with its publication pending

submitter keyword: Human, Dendritic cell, vaccine, newborn, RSV

Simon van Haren
contact affiliation	1.Precision Vaccines Program, Division of Infectious Diseases, Boston Children’s Hospital, Boston, MA, USA. 2. Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
contact email	simon.vanharen@childrens.harvard.edu
lab head
Simon van Haren
contact affiliation	Boston Children's Hospital
contact email	simon.vanharen@childrens.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025568
     1. Label: PRIDE project
     2. Name: A single immunization with CAF08 provides newborns with Th1-mediated protection against Respiratory Syncytial Virus infection