PXD025556

PXD025556 is an original dataset announced via ProteomeXchange.

Title	Characterization and therapeutic repercussion of the ecology in HER2 positive breast cancer
Description	Conditioned medium experiments carried out in our laboratory with CAFs derived from HER2-positive patients showed a significant capacity to promote resistance to trastuzumab plus pertuzumab therapies in two HER2-positive breast cancer cell lines (BCCLs), even in the presence of docetaxel. In order to elucidate the components of CAF-conditioned medium that would be relevant in the promotion of BCCL resistance, we performed a multi-omics strategy to identify miRNAs, cytokines, transcription factors and/or kinases in the secretome that target specific objectives in cancer cells. The combination of miRNA analysis, label-free LC-MS/MS quantification and cytokine arrays to explore the secretome of CAFs under treatment conditions revealed several up- and down-regulated candidates. We discuss the potential role of some of the most interesting candidates in generating resistance in HER2-positive breast cancer
HostingRepository	PRIDE
AnnounceDate	2022-01-26
AnnouncementXML	Submission_2022-01-26_06:50:44.458.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Concha Gil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-22 04:09:36	ID requested
⏵ 1	2022-01-26 06:50:44	announced

Dataset with its publication pending

submitter keyword: Breast cancer

HER2-positive

tumour microenvironment

targeted-therapy

trastuzumab

resistance

cancer-associated fibroblast

label-free proteomics

Juan Madoz-Gúrpide
contact affiliation	Pathology, Fundación Jiménez Díaz University Hospital Health Research Institute (IIS—FJD, UAM), Madrid 28040, Spain
contact email	JMadoz@fjd.es
lab head
Concha Gil
contact affiliation	UMC
contact email	cai.proteomica@pas.ucm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD025556

PRIDE project URI

• PRIDE
  1. PXD025556
     1. Label: PRIDE project
     2. Name: Characterization and therapeutic repercussion of the ecology in HER2 positive breast cancer