PXD025548

PXD025548 is an original dataset announced via ProteomeXchange.

Title	Stable isotope-resolved metabolomics reveals the abnormal brain glucose catabolism in depression based on chronic unpredictable mild stress rats
Description	The severe harm of depression to human life has attracted great attention to neurologists, but its pathogenesis is extremely complicated and has not yet been fully elaborated. Here, we provided a new strategy for revealing the specific pathways of abnormal brain glucose catabolism in depression, which from the supply of energy substrates and the evaluation of mitochondrial structure and function. By using stable isotope-resolved metabolomics technique, we discovered the tricarboxylic acid cycle (TCA cycle) is blocked and the gluconeogenesis is abnormally activated in chronic unpredictable mild stress (CUMS) rats. In addition, our results showed an interesting phenomenon that the brain attempted to activate all possible metabolic enzymes in energy-producing pathways, but CUMS rats still exhibited a low TCA cycle activity due to impaired mitochondria. Depression caused mitochondrial structure and function impaired, and then led to abnormal brain glucose catabolism. The combination of the stable isotope-resolved metabolomics and mitochondrial structure and function analysis can accurately clarify the mechanism of depression. The mitochondrial pyruvate carrier and acetyl-CoA maybe the key targets for depression treatment. The strategy provides a unique insight for exploring the mechanism of depression, the discovery of new targets, and the development of ideal novel antidepressants.
HostingRepository	PRIDE
AnnounceDate	2021-06-10
AnnouncementXML	Submission_2021-06-10_00:50:59.928.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting Linghu
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-22 00:25:21	ID requested
⏵ 1	2021-06-10 00:51:00	announced

Ling-Hu T, Liu SB, Gao Y, Han YM, Tian JS, Qin XM, Stable Isotope-Resolved Metabolomics Reveals the Abnormal Brain Glucose Catabolism in Depression Based on Chronic Unpredictable Mild Stress Rats. J Proteome Res, 20(7):3549-3558(2021) [pubmed]

submitter keyword: depression, brain glucose catabolism, stable isotope-resolved metabolomics, liquid chromatography-mass spectrometry, mitochondrial structure and function

Xuemei Qin
contact affiliation	Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, Shanxi, China
contact email	qinxm@sxu.edu.cn
lab head
Ting Linghu
contact affiliation	Modern Research Center for Traditional Chinese Medicine
contact email	linghuting1992@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025548
     1. Label: PRIDE project
     2. Name: Stable isotope-resolved metabolomics reveals the abnormal brain glucose catabolism in depression based on chronic unpredictable mild stress rats