PXD025535

PXD025535 is an original dataset announced via ProteomeXchange.

Title	A multifactorial proteomics approach to sex-specific effects of diet composition and social environment in an omnivorous insect
Description	Rearing conditions may elicit noticeable plastic responses in life-history traits of living organisms. For instance, diet composition has proven to influence prominent traits such as body size, fecundity, and lifespan. In contrast, the social environment may influence the survival rate at immature stages, food consumption, and fat storage. Nevertheless, the physiological mechanisms underlying such responses are largely unknown. In this study, we investigated changes in the proteome of the house cricket Acheta domesticus subjected to diets of different nutritional composition (i.e., protein to carbohydrates ratio) and two distinct social environments (i.e., solitary or in groups). We measured the relative abundances of 685 proteins identified in whole-body cricket samples using high-performance liquid chromatography coupled to tandem mass spectrometry. Differential expression of proteins induced by diet composition and social environment in female and male A. domesticus was assessed in a data-independent proteomics approach. Additionally, we performed a functional analysis of the differentially expressed proteins using comprehensive databases (KEGG and GO). We found that sex alone explained a significant portion (40.87%) of the relative protein abundance variation. Males had a higher representation of proteins involved in metabolic pathways and locomotion. In contrast, females exhibited a higher abundance of proteins related to genetic processes regulation and nutrient catabolism. Moreover, diet composition and social environment induced sex-specific changes in a smaller set of proteins with particular roles. Females had their protein profile affected by diet composition and social environment. The involved proteins were mainly related to several protein synthesis stages, carbohydrate metabolism, and muscle development. In contrast, males were only affected by diet composition, overexpressing proteins related to hormone production, carbohydrate metabolism, and apparently depositing excess protein in the cuticle when fed with a protein-rich diet. It was evident that diet had a more substantial influence on the proteome of the cricket A. domesticus than the social environment, showing that diet composition may exert profound physiological changes in insects in a sex-specific manner.
HostingRepository	PRIDE
AnnounceDate	2021-05-31
AnnouncementXML	Submission_2021-05-31_07:03:05.077.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marion Fresch
SpeciesList	scientific name: Acheta domesticus; NCBI TaxID: 6997;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-21 07:47:56	ID requested
⏵ 1	2021-05-31 07:03:05	announced

Dataset with its publication pending

submitter keyword: liquid chromatography, tandem mass spectrometry, SWATH, IDA, differential expression, Acheta domesticus, nutritional ecology, multifactorial experiment, house cricket

Prof. Dr. Jens Brockmeyer
contact affiliation	Department of Food Chemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
contact email	jens.brockmeyer@lc.uni-stuttgart.de
lab head
Marion Fresch
contact affiliation	University of Stuttgart, Institute of Biochemistry and Technical Biochemistry, Department of Food Chemistry
contact email	marion.fresch@lc.uni-stuttgart.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025535
     1. Label: PRIDE project
     2. Name: A multifactorial proteomics approach to sex-specific effects of diet composition and social environment in an omnivorous insect