PXD025515

PXD025515 is an original dataset announced via ProteomeXchange.

Title	TMT-based quantitative proteomics of exosomes from porcine colostrum and milk
Description	Colostrum and milk have high nutritional value and provide a complete diet for neonates, along with bioactive substances which modulate various functions such as immune defense. Exosomes are membranous vesicles of endocytic origin, recently been considered as major players in cell-cell communication. The mechanisms by which milk components can prime the infant’s active immunity are not entirely clear, and exosomes are suggested to be essential for the infant’s physiological development. We assessed the exosomal proteome profile from milk samples obtained from 10 healthy sows, at day 0 (colostrum), day 7, and 14 post-partum. Exosomes were isolated by ultracentrifugation coupled with size exclusion chromatography, and were characterized by nanoparticle tracking analysis, transmission electron microscopy and Western blotting for exosome markers. Isolated exosomes were in-gel digested and after TMT-labelling of the peptides, they were subjected to LC-MS/MS. Quantitative proteomics analysis revealed different proteome profiles for colostrum exosomes and milk exosomes, and functional analysis highlighted pathways related to immune response, cellular development, and regulation of cellular processes. This study endorses the importance of exosomes as active biocomponents of milk and provides knowledge for future studies exploring their role in regulation of immunity and growth of the newborn.
HostingRepository	PRIDE
AnnounceDate	2022-02-08
AnnouncementXML	Submission_2022-02-08_04:52:40.263.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025515
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marc Sylvester
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-21 02:42:34	ID requested
⏵ 1	2022-02-08 04:52:41	announced

Ferreira RF, Blees T, Shakeri F, Buness A, Sylvester M, Savoini G, Agazzi A, Mrljak V, Sauerwein H, Comparative proteome profiling in exosomes derived from porcine colostrum versus mature milk reveals distinct functional proteomes. J Proteomics, 249():104338(2021) [pubmed]

submitter keyword: orcine, exosomes, extracellular vesicles, LC-MS/MS, TMT labelling, milk, colostrum, pig

Helga Sauerwein
contact affiliation	University of Bonn, Germany
contact email	sauerwein@uni-bonn.de
lab head
Marc Sylvester
contact affiliation	Institute of Biochemistry and Molecular Biology, Medical Faculty, University of Bonn
contact email	msylvest@uni-bonn.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025515
     1. Label: PRIDE project
     2. Name: TMT-based quantitative proteomics of exosomes from porcine colostrum and milk