PXD025510

PXD025510 is an original dataset announced via ProteomeXchange.

Title	Huntingtin polyglutamine expansion modulates protein interaction dynamics in the striatum
Description	Huntington’s disease is a monogenic disorder, with only one known causative gene, huntingtin (HTT). Expansion of a trinucleotide (CAG) repeat within the HTT gene results in a mutant protein containing polyglutamine (polyQ) stretches. While mutant HTT is the primary driver of cellular degeneration in the brain, the molecular and cellular mechanisms are incompletely understood. To further understand the role of polyQ in disease pathogenesis, we studied the dynamics of HTT protein-protein interactions (PPIs) in the striatum of mice with wild-type (Q20) and mutant (Q140) HTT at pre-symptomatic and manifest HD ages using label-free and isotope-labeled IP-MS approaches. Combining these approaches, we demonstrated that HTT PPI dynamics are distinct in early and later disease phases. Computational analysis pointed to early protein interaction changes involving synaptic transmission and vesicle fusion functions, while later interactions underlie changes in synapse morphogenesis and impact the actin cytoskeletal network.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:54.575.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Todd Greco
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-20 23:19:03	ID requested
1	2022-02-20 09:59:04	announced
⏵ 2	2023-11-14 08:52:55	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Orbitrap Velos,IP-MS, HTT, LFQ, IDIRT

Ileana Cristea
contact affiliation	Department of Molecular Biology, Princeton University, Princeton NJ, USA
contact email	icristea@princeton.edu
lab head
Todd Greco
contact affiliation	Molecular Biology, Princeton University
contact email	tgreco@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025510

PRIDE project URI

• PRIDE
  1. PXD025510
     1. Label: PRIDE project
     2. Name: Huntingtin polyglutamine expansion modulates protein interaction dynamics in the striatum