PXD025496 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FCGBP, the IgGFc-binding protein, is secreted with all GDPH sequences cleaved, but maintained by inter-fragment disulfide bonds |
Description | Mucus has an important protective function and forms a barrier that minimizes the bacterial contact with the colonic epithelium. It is organized as a complex network with several specific proteins of which most are still poorly understood. Abundant proteins in intestinal mucus, in addition to the mucin MUC2, are CLCA1 and FCGBP. FCGBP is expressed in all intestinal goblet cells and secreted into the mucus. It is comprised of repeated von Willebrand Factor D (vWD) domain assemblies, of which most have a GDPH amino acid sequence. This sequence can be autocatalytically cleaved, as previously found in MUC2 and MUC5AC. We show that all vWD with a GDPH sequence are cleaved, and that these cleavages occur early during biosynthesis in the endoplasmic reticulum. All cleaved fragments remained connected by a disulfide bond within each vWD domain. The cleavage can generate a C-terminal reactive Asp-anhydride that could react with other molecules, such as MUC2, but this was not observed. Quantitative analyses by mass spectrometry showed that, in chaotropic solutions, FCGBP was mainly soluble whereas MUC2 was insoluble, and most of the secreted FCGBP was not covalently bound to MUC2. As its name indicates, FCGBP has been suggested to bind IgG, something that was not possible to reproduce in vitro using purified proteins. The function of FCGBP is still unknown, but our results suggest that the Asp-anhydrides do not contribute to covalent crosslinking in the mucin framework nor does FCGBP incorporate IgG in mucus. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-09 |
AnnouncementXML | Submission_2021-06-09_09:07:25.680.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Erik Ehrencrona |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | LTQ Orbitrap XL |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-20 06:33:43 | ID requested | |
⏵ 1 | 2021-06-09 09:07:26 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: FCGBP, IgG, GDPH, Mucus |
Contact List
Malin E.V. Johansson |
contact affiliation | Instution of Biomecine, Department of Medical Biochemistry, University of Gothenburg |
contact email | malin.johansson@medkem.gu.se |
lab head | |
Erik Ehrencrona |
contact affiliation | Gothenburg University |
contact email | erik.ehrencrona@medkem.gu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD025496
- Label: PRIDE project
- Name: FCGBP, the IgGFc-binding protein, is secreted with all GDPH sequences cleaved, but maintained by inter-fragment disulfide bonds