PXD025489 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis |
Description | Some carboxydotrophs like Rhodospirillum rubrum are able to grow with CO as their sole source of energy using a Carbone monoxide dehydrogenase (CODH) and an Energy conserving hydrogenase (ECH) to perform anaerobically the so called water-gas shift reaction (WGSR) (CO + H2O-> CO2 + H2). Several studies have focused at the biochemical and biophysical level on this enzymatic system and a few OMICS studies on CO metabolism. Knowing that CO is toxic in particular due to its binding to heme iron atoms, and is even considered as a potential antibacterial agent, we decided to use a proteomic approach in order to analyze R. rubrum adaptation in term of metabolism and management of the toxic effect. In particular, this study allowed highlighting a set of proteins likely implicated in ECH maturation, and important perturbations in term of cofactor biosynthesis, especially metallic cofactors. This shows that even this CO tolerant microorganism cannot avoid completely CO toxic effects associated with its interaction with metallic ions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-09 |
AnnouncementXML | Submission_2024-09-09_01:58:35.478.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD025489 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Hélène Diemer |
SpeciesList | scientific name: Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255); NCBI TaxID: 269796; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-20 02:16:48 | ID requested | |
⏵ 1 | 2024-09-09 01:58:35 | announced | |
Publication List
Keyword List
submitter keyword: shotgun proteomics,Rhodospirillum rubrum, LC-MS/MS |
Contact List
Cianferani Sarah |
contact affiliation | Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg,CNRS, IPHC UMR 7178, 67087 Strasbourg,France |
contact email | sarah.cianferani@unistra.fr |
lab head | |
Hélène Diemer |
contact affiliation | LSMBO |
contact email | hdiemer@unistra.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025489
- Label: PRIDE project
- Name: Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis