PXD025489

PXD025489 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis
Description	Some carboxydotrophs like Rhodospirillum rubrum are able to grow with CO as their sole source of energy using a Carbone monoxide dehydrogenase (CODH) and an Energy conserving hydrogenase (ECH) to perform anaerobically the so called water-gas shift reaction (WGSR) (CO + H2O-> CO2 + H2). Several studies have focused at the biochemical and biophysical level on this enzymatic system and a few OMICS studies on CO metabolism. Knowing that CO is toxic in particular due to its binding to heme iron atoms, and is even considered as a potential antibacterial agent, we decided to use a proteomic approach in order to analyze R. rubrum adaptation in term of metabolism and management of the toxic effect. In particular, this study allowed highlighting a set of proteins likely implicated in ECH maturation, and important perturbations in term of cofactor biosynthesis, especially metallic cofactors. This shows that even this CO tolerant microorganism cannot avoid completely CO toxic effects associated with its interaction with metallic ions.
HostingRepository	PRIDE
AnnounceDate	2024-09-09
AnnouncementXML	Submission_2024-09-09_01:58:35.478.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025489
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hélène Diemer
SpeciesList	scientific name: Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255); NCBI TaxID: 269796;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-20 02:16:48	ID requested
⏵ 1	2024-09-09 01:58:35	announced

10.6019/PXD025489;

10.1016/J.JPROT.2021.104389;

submitter keyword: shotgun proteomics,Rhodospirillum rubrum, LC-MS/MS

Cianferani Sarah
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg,CNRS, IPHC UMR 7178, 67087 Strasbourg,France
contact email	sarah.cianferani@unistra.fr
lab head
Hélène Diemer
contact affiliation	LSMBO
contact email	hdiemer@unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025489
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Rhodospirillum rubrum after carbon monoxide exposure reveals an important effect on metallic cofactor biosynthesis