PXD025475

PXD025475 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of exosomes isolated from invasive and proliferative melanoma cell lines and correlation with exosomes from plasma of human melanoma patients pre- and post-checkpoint inhibitor treatment
Description	Over the last decades, exosomes have received increasing attention due to their involvement in numerous pathologies including cancer. Tumor-derived exosomes and exosomes derived from the tumor microenvironment are implicated in multiple mechanisms that support disease progression such as the escape of malignant cells from immunosurveillance, tumor cell growth, tumor angiogenesis, preparation of a pre-metastatic niche and remodeling of the extracellular matrix, thereby promoting dissemination and metastasis. Here, we performed protein expression phenotyping of exosomes derived from different invasive and proliferative melanoma cell lines (n=8) to provide a solid framework of gene expression programs, which - in a clinical setting - would be useful for prognosis and may also predict treatment response. Cell line characteristics have been published previously (Wenzina et al.,2020). Having identified a set of differentially expressed proteins in proliferative and invasive melanoma cell lines, we correlated them to the protein composition of plasma exosomes from melanoma patients pre and post immunotherapy treatment (n=7) as well as healthy controls (n=5).
HostingRepository	PRIDE
AnnounceDate	2022-07-14
AnnouncementXML	Submission_2022-07-14_09:06:22.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marianne Schnödl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetic acid derivatized residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-19 07:49:52	ID requested
⏵ 1	2022-07-14 09:06:22	announced

Dataset with its publication pending

submitter keyword: Melanoma, Cell culture, Plasma, Exosomes, LC-MSMS

Peter Petzelbauer
contact affiliation	Medical University of Vienna / Department of Dermatology / Head of Skin & Endothelium Research Division (SERD)
contact email	peter.petzelbauer@meduniwien.ac.at
lab head
Marianne Schnödl
contact affiliation	Medical University of Vienna / Department of Dermatology / Skin & Endothelium Research Division (SERD)
contact email	marianne.schnoedl@meduniwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD025475

PRIDE project URI

• PRIDE
  1. PXD025475
     1. Label: PRIDE project
     2. Name: Proteomic profiling of exosomes isolated from invasive and proliferative melanoma cell lines and correlation with exosomes from plasma of human melanoma patients pre- and post-checkpoint inhibitor treatment