PXD025469

PXD025469 is an original dataset announced via ProteomeXchange.

Title	Quantification of Peptide 2 (RLLWENGNL) concentration in the mouse plasma
Description	Diabetes mellitus (DM) causes the change in the components of the plasma, which may induce tissue and organ injuries, including the heart and kidney. In the diabetic model db/db mice, intravenous injection of purified dipeptidyl peptidase III (DPPIII) attenuated the heart and kidney damages provoked by DM without alteration in blood glucose level. This led us to hypothesize that there might be peptide(s) that are involved in the progression of the DM-mediated damages and are cleaved by DPPIII. To explore the peptides, the whole peptidomics analysis (shotgun peptidomics) was conducted using tandem mass spectrometry, in which peptides derived from the plasma of PBS-infused C57BL/6 mice, PBS-infused db/db mice, and DPPIII-treated db/db mice were compared. The raw mass spectrometry data were deposited to the ProteomeXchange with the dataset identifier PXD025440. Together with the mass spectrometry analysis and other experiments, we identified a peptide named Peptide 2 (AA sequence: RLLWENGNL) as a substrate of DPPIII. Peptide 2 is a part of C3f (53% identical), and C3f can act as an anaphylatoxin like C3a. To quantify the amount in plasma derived from the plasma of PBS-infused C57BL/6 mice, PBS-infused db/db mice, and DPPIII-treated db/db mice, selected reaction monitoring (SRM) assay was performed. All of the chromatograms and quantified data obtained by SRM from the three mouse groups were shown in the Analyst formated and PDF files.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_00:40:39.332.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akio Shimizu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-19 06:31:29	ID requested
⏵ 1	2022-02-17 00:40:40	announced

Komeno M, Pang X, Shimizu A, Molla MR, Yasuda-Yamahara M, Kume S, Rahman NIA, Soh JEC, Nguyen LKC, Ahmat Amin MKB, Kokami N, Sato A, Asano Y, Maegawa H, Ogita H, Cardio- and reno-protective effects of dipeptidyl peptidase III in diabetic mice. J Biol Chem, 296():100761(2021) [pubmed]

submitter keyword: mouse, plasma, peptide, peptidase, diabetes mellitus, selected reaction monitoring, SRM

Hisakazu Ogita
contact affiliation	Shiga University of Medical Science
contact email	hogita@belle.shiga-med.ac.jp
lab head
Akio Shimizu
contact affiliation	Shiga University of Medical Science
contact email	shimizua@belle.shiga-med.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025469
     1. Label: PRIDE project
     2. Name: Quantification of Peptide 2 (RLLWENGNL) concentration in the mouse plasma