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PXD025436

PXD025436 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSurface-shaving of Staphylococcus aureus strains using Lipid-based Protein Immobilization (LPI) and quantitative proteomic analysis reveal differences in protein expression levels of the surfaceome
DescriptionStaphylococcus aureus is a pathogen which can cause a wide range of infections. To find new targets for diagnostics and treatment as well as understanding host-pathogen interactions, many studies have focused on the bacterial surface proteins. In the study presented here, bacterial cell surface-shaving, using Lipid-based Protein Immobilization (LPI), followed by tandem mass tag (TMT) protocols for performing relative quantitative mass spectrometry proteomics, was performed to examine the surface proteome (surfaceome) of selected strains of S. aureus. The study included analyses of surface protein-deficient mutants compared to the wildtype S. aureus strain Newman, as well strain isolates of different clinical associations. Quantitative proteomics were applied in the analysis of the surface proteome of Staphylococcus aureus. In study 1, the differential abundance of proteins in the mutant strains ΔClfA, ΔSrtAΔSrtB and ΔSpa was compared to those in the Newman parental strain. All together 7880 peptides were identified in Study 1 corresponding to 1290 proteins, and the results clearly showed that the mutant strains were deficient in the knocked-out genes. The Clinical strains study (Study 2) included the Newman strain, the reference strains LS-1 and SH1000 and three clinical isolates, two from invasive infections and one from mild skin infection. A total of 4949 peptides were identified in Study 2 corresponding to 919 proteins. For each strain, approximately 20 proteins showed higher or lower abundance (fold changes) when compared to the Newman strain. The results indicate that surface shaving of intact bacteria by LPI in combination with protocols for performing quantitative proteomics makes it possible to distinguish differences in protein abundance of the surfaceome, including virulence factors, between S. aureus strains.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_03:14:04.430.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterProteomics Core Facility
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-16 03:50:34ID requested
12024-10-17 03:14:06announced
Publication List
10.3390/microorganisms12081725;
Karlsson A, Alarc, ó, n LA, Pi, ñ, eiro-Iglesias B, Jacobsson G, Skovbjerg S, Moore ERB, Kopparapu PK, Jin T, Karlsson R, Strains and Quantitative Proteomic Analysis Reveal Differences in Protein Abundance of the Surfaceome. Microorganisms, 12(8):(2024) [pubmed]
Keyword List
submitter keyword: proteomics, relative quantification, mass spectrometry, virulence factors,Staphylococcus aureus, surfaceome, tandem mass tags
Contact List
Roger Karlsson
contact affiliationDepartment of Infection Diseases, Institute of Biomedicine,Sahlgrenska Academy of the University of Gothenburg
contact emailroger.karlsson@nanoxisconsulting.com
lab head
Proteomics Core Facility
contact affiliationSAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact emailgupcf@outlook.com
dataset submitter
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Dataset FTP location
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