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PXD025431

PXD025431 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAtopic dermatitis epidermis PCT-DIA
DescriptionAtopic Dermatitis (AD) is the most common inflammatory skin disease and characterized by a deficient epidermal barrier and cutaneous inflammation. Genetic studies suggest a key role of keratinocytes in AD pathogenesis, but the alterations in the proteome that occur in the entire epidermis have not been defined. Employing a pressure-cycling technology-data-independent acquisition (PCT-DIA) approach, we performed quantitative proteomics of epidermis from healthy volunteers and lesional and non-lesional skin of AD patients. Results were validated by targeted proteomics using parallel reaction monitoring mass spectrometry or by immunofluorescence staining. The identified proteins reflect the strong inflammation in lesional skin and the defect in keratinocyte differentiation and epidermal stratification. Most importantly, they reveal impaired activation of the NRF2-antioxidant pathway and reduced abundance of mitochondrial proteins involved in key metabolic pathways in the epidermis. These results provide insight into the molecular alterations in the epidermis of AD patients and identify novel targets for pharmaceutical intervention.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:11:35.691.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUlrich auf dem Keller
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-15 22:26:13ID requested
12022-10-04 04:20:36announced
22023-11-14 08:11:36announced2023-11-14: Updated project metadata.
Publication List
Koch M, Kockmann T, Rodriguez E, Wehkamp U, Hiebert P, Ben-Yehuda Greenwald M, St, รถ, lzl D, Beer HD, Tschachler E, Weidinger S, Werner S, Auf dem Keller U, Quantitative Proteomics Identifies Reduced NRF2 Activity and Mitochondrial Dysfunction in Atopic Dermatitis. J Invest Dermatol, 143(2):220-231.e7(2023) [pubmed]
Keyword List
submitter keyword: pressure cycling technology,atopic dermatitis, data-independent acquisition, epidermis
Contact List
Ulrich auf dem Keller
contact affiliationTechnical University of Denmark, Department of Biotechnology and Biomedicine ETH Zurich, Department of Biology
contact emailuadk@dtu.dk
lab head
Ulrich auf dem Keller
contact affiliationTechnical University of Denmark
contact emailulrich.aufdemkeller@biol.ethz.ch
dataset submitter
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Dataset FTP location
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