PXD025431 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Atopic dermatitis epidermis PCT-DIA |
Description | Atopic Dermatitis (AD) is the most common inflammatory skin disease and characterized by a deficient epidermal barrier and cutaneous inflammation. Genetic studies suggest a key role of keratinocytes in AD pathogenesis, but the alterations in the proteome that occur in the entire epidermis have not been defined. Employing a pressure-cycling technology-data-independent acquisition (PCT-DIA) approach, we performed quantitative proteomics of epidermis from healthy volunteers and lesional and non-lesional skin of AD patients. Results were validated by targeted proteomics using parallel reaction monitoring mass spectrometry or by immunofluorescence staining. The identified proteins reflect the strong inflammation in lesional skin and the defect in keratinocyte differentiation and epidermal stratification. Most importantly, they reveal impaired activation of the NRF2-antioxidant pathway and reduced abundance of mitochondrial proteins involved in key metabolic pathways in the epidermis. These results provide insight into the molecular alterations in the epidermis of AD patients and identify novel targets for pharmaceutical intervention. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:11:35.691.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ulrich auf dem Keller |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-15 22:26:13 | ID requested | |
1 | 2022-10-04 04:20:36 | announced | |
⏵ 2 | 2023-11-14 08:11:36 | announced | 2023-11-14: Updated project metadata. |
Publication List
Koch M, Kockmann T, Rodriguez E, Wehkamp U, Hiebert P, Ben-Yehuda Greenwald M, St, รถ, lzl D, Beer HD, Tschachler E, Weidinger S, Werner S, Auf dem Keller U, Quantitative Proteomics Identifies Reduced NRF2 Activity and Mitochondrial Dysfunction in Atopic Dermatitis. J Invest Dermatol, 143(2):220-231.e7(2023) [pubmed] |
Keyword List
submitter keyword: pressure cycling technology,atopic dermatitis, data-independent acquisition, epidermis |
Contact List
Ulrich auf dem Keller |
contact affiliation | Technical University of Denmark, Department of Biotechnology and Biomedicine ETH Zurich, Department of Biology |
contact email | uadk@dtu.dk |
lab head | |
Ulrich auf dem Keller |
contact affiliation | Technical University of Denmark |
contact email | ulrich.aufdemkeller@biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD025431 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025431
- Label: PRIDE project
- Name: Atopic dermatitis epidermis PCT-DIA