PXD025423

PXD025423 is an original dataset announced via ProteomeXchange.

Title	Long-term exposure to nanosized TiO2 triggers stress responses and cell death pathways in pulmonary epithelial cells
Description	There is little in vitro data available on long-term effects of TiO2 exposure. Such data are im-portant for improving the understanding of underlying mechanisms of adverse health effects of TiO2. Here, we exposed pulmonary epithelial cells to two doses (0.96 and 1.92 µg/cm2) of TiO2 for 13 weeks and effects on cell cycle and cell death mechanisms i.e. apoptosis and autophagy were determined after 4, 8 and 13 weeks of exposure. Changes in telomere length, cellular protein levels and lipid classes were also analysed at 13 weeks of exposure. We observed that the TiO2 exposure increased the fraction of cells in G1-phase and reduced the fraction of cells in G2-phase, which was accompanied by an increase in the fraction of late apoptotic/necrotic cells. This cor-responded with an induced expression of key apoptotic proteins i.e. BAD and BAX, and an ac-cumulation of several lipid classes involved in cellular stress and apoptosis. These findings were further supported by quantitative proteome profiling data showing an increase in proteins in-volved in cell stress and genomic maintenance pathways following TiO2 exposure. Altogether, we suggest that cell stress response and cell death pathways may be important molecular events in long-term health effects of TiO2.
HostingRepository	PRIDE
AnnounceDate	2021-07-15
AnnouncementXML	Submission_2021-07-15_02:59:53.699.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-15 04:57:32	ID requested
⏵ 1	2021-07-15 02:59:54	announced

Alswady-Hoff M, Erdem JS, Phuyal S, Knittelfelder O, Sharma A, Fonseca DM, Skare Ø, Slupphaug G, Zienolddiny S, Triggers Stress Responses and Cell Death Pathways in Pulmonary Epithelial Cells. Int J Mol Sci, 22(10):(2021) [pubmed]

submitter keyword: Titanium dioxide

Fibrosis

Carcinogenesis

Proteomics

Lipidomics

Mayes Alswady-Hoff
contact affiliation	National Institute of Occupational Health, Pb 8149 Dep, N-0033, Oslo, Norway
contact email	Mayes.Alswady-Hoff@stami.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025423

PRIDE project URI

• PRIDE
  1. PXD025423
     1. Label: PRIDE project
     2. Name: Long-term exposure to nanosized TiO2 triggers stress responses and cell death pathways in pulmonary epithelial cells