PXD025412 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of ESAG4-interacting proteins in Trypanosoma brucei bloodstream forms |
Description | How flagellar signaling regulates the host interaction of parasites remains a challenge due to poor conservation of signaling systems with those in cilia of higher organisms. Flagellar cAMP signaling is implicated in interaction of the unicellular parasite Trypanosoma brucei with its hosts. The second messenger is produced by a large and atypical family of receptor-type adenylate cyclases (ACs) with unknown regulation mechanism. In order to identify trypanosome AC interacting proteins, ESAG4, an AC highly abundant in the parasite’s bloodstream stage, was fused to GFP and purified via GFP trap pull down followed by mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-31 |
AnnouncementXML | Submission_2022-08-31_11:02:49.678.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ignasi Forne |
SpeciesList | scientific name: Trypanosoma brucei brucei; NCBI TaxID: 5702; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-15 01:20:42 | ID requested | |
⏵ 1 | 2022-08-31 11:02:50 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: adenylate cyclase, cyclase-interacting protein, cAMP signaling, GFP trap |
Contact List
Boshart, Michael |
contact affiliation | Biocenter, Faculty of Biology, Genetics Ludwig-Maximilians-University Munich (LMU) 82152 Martinsried, Germany |
contact email | boshart@lmu.de |
lab head | |
Ignasi Forne |
contact affiliation | Biomedical Center-LMU |
contact email | ignasi.forne@lrz.uni-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD025412 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025412
- Label: PRIDE project
- Name: Identification of ESAG4-interacting proteins in Trypanosoma brucei bloodstream forms