PXD025411
PXD025411 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum |
Description | Diplonema papillatum represents a group of highly diverse and abundant marine protists with still unknown lifestyle and ecological functions. Based on alterations of the transcriptomic, proteomic and metabolomic profiles obtained from cells grown under different conditions we designed a metabolic map of its cellular bioenergetic pathways. Comparative analysis in the nutrient-rich and -poor media and in the absence and presence of oxygen revealed a capacity for major metabolic reprograming. D. papillatum is equipped with fundamental metabolic routes such as glycolysis, gluconeogenesis, TCA cycle, pentose phosphate pathway, respiratory complexes, β-oxidation and synthesis of fatty acid. While gluconeogenesis uniquely dominates over glycolysis, TCA cycle represents a combination of standard and unusual enzymes. The presence of typical anaerobic enzymes such as pyruvate:NADP+ oxidoreductase, fumarate reductase, opine dehydrogenase, enoyl-coenzyme A reductase, and lactate dehydrogenase reflects the ability to survive in low-oxygen environments. The metabolism quickly reacts to restricted carbon source, revealing unusual flexibility of diplonemids, also reflected in cell morphology and motility, which is in good correlation with their extreme ecological valence. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-28 |
AnnouncementXML | Submission_2023-07-28_04:23:08.493.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD025411 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | PeterBarath |
SpeciesList | scientific name: Diplonema papillatum; NCBI TaxID: 91374; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-04-15 01:20:41 | ID requested | |
1 | 2021-11-15 03:34:39 | announced | |
⏵ 2 | 2023-07-28 04:23:09 | announced | 2023-07-28: Updated project metadata. |
Publication List
Š, kodov, á, -Sver, á, kov, á I, Z, á, honov, á K, Juricov, á V, Danchenko M, Moos M, Bar, á, th P, Prokopchuk G, Butenko A, Luk, á, č, ov, á V, Koh, ú, tov, á L, Bu, č, kov, á B, Hor, á, k A, Faktorov, á D, Horv, á, th A, Š, imek P, Luke, š J, Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum. BMC Biol, 19(1):251(2021) [pubmed] |
Keyword List
submitter keyword: metabolism, euglenozoans,Diplonema papillatum, hypoxia, mitochondrion |
Contact List
PeterBarath | |
---|---|
contact affiliation | Institute of Chemistry, Slovak Academy of Sciences |
contact email | peter.barath@savba.sk |
lab head | |
PeterBarath | |
contact affiliation | Institute of Chemistry, Slovak Academy of Sciences |
contact email | peter.barath@savba.sk |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025411 |
PRIDE project URI |
Repository Record List
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