PXD025397

PXD025397 is an original dataset announced via ProteomeXchange.

Title	Combination of a microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids
Description	Brain organoids derived from human pluripotent stem cells provide a highly valuable in vitro model to recapitulate human brain development and neurological diseases. However, the current systems for brain organoid culture require further improvement for the reliable production of high-quality organoids. Here, we demonstrate two engineering elements to improve human brain organoid culture, (1) a human brain extracellular matrix (BEM) to provide brain-specific cues and (2) a microfluidic device with periodic flow to improve the survival and reduce the variability of organoids. A three-dimensional culture modified with BEM significantly enhanced neurogenesis in developing brain organoids from human induced pluripotent stem cells. Cortical layer development, volumetric augmentation, and electrophysiological function of human brain organoids were further improved in a reproducible manner by dynamic culture in microfluidic chamber devices. Our engineering concept of reconstituting brain-mimetic microenvironments facilitates the development of a reliable culture platform for brain organoids, enabling effective modeling and drug development for human brain diseases.
HostingRepository	PRIDE
AnnounceDate	2021-06-02
AnnouncementXML	Submission_2021-08-26_04:38:35.151.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sung-Hyun Jo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-14 06:10:57	ID requested
1	2021-06-02 00:26:53	announced
⏵ 2	2021-08-26 04:38:36	announced	2021-08-26: Updated publication reference for PubMed record(s): 34354063.

Cho AN, Jin Y, An Y, Kim J, Choi YS, Lee JS, Kim J, Choi WY, Koo DJ, Yu W, Chang GE, Kim DY, Jo SH, Kim J, Kim SY, Kim YG, Kim JY, Choi N, Cheong E, Kim YJ, Je HS, Kang HC, Cho SW, Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids. Nat Commun, 12(1):4730(2021) [pubmed]

submitter keyword: brain extracellular matrix, microfluidic device, organoid

Seung-Woo Cho
contact affiliation	Department of Biotechnology, Stem cell and biomaterial engineering Lab, Yonsei University, Republic of Korea.
contact email	seungwoocho@yonsei.ac.kr
lab head
Sung-Hyun Jo
contact affiliation	Soongsil university
contact email	sh20511@soongsil.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025397

PRIDE project URI

• PRIDE
  1. PXD025397
     1. Label: PRIDE project
     2. Name: Combination of a microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids