PXD025390

PXD025390 is an original dataset announced via ProteomeXchange.

Title	Maternal Adiponectin Decreases Placenta NutrientTransport in Mice
Description	Obese women who develop gestational diabetes show lower adiponectin levels across pregnancy than obese euglycemic women suggesting that obese women with low adiponectin levels have an impaired capacity to handle the metabolic changes during pregnancy. Adiponectin acts on the placenta during pregnancy; this fact allows for the interesting possibility that adiponectin can exert endocrine effects on the developing fetus. The aim was to investigate how adiponectin affects fetal growth, placenta function and metabolic functions during pregnancy. Wild-type (wt) and adiponectin transgenic (APNtg) mice were fed normal chow or a high fat/high sucrose (HF/HS) diet for 8 weeks before mating. Dams and fetuses were dissected at gestational day 18.5. Maternal adiponectin overexpression decreased fetal body weight in dams on normal chow, and even more in dams on HF/HS diet. Pools of two placentas or two livers from one male and one female fetus with the same genotype (wt or APNtg) from the same dam on HF/HS diet were used in proteomic analysis, and a total of five pools per group (wt-wt, wt-APNtg, APNtg-wt, APNtg-APNtg) were included in the liquid chromatography-tandem mass spectrometry (LS-MS/MS) analysis. In total, 7290 proteins were identified in placenta, and 7301 proteins in fetal liver. Next, we analyzed phosphosites, in total,19264 sites were identified in placenta proteins and 15334 sites were identified in fetal liver proteins. We then applied pathway analysis to the total protein and phosphopeptide data using PRISM and Enrichr.
HostingRepository	PRIDE
AnnounceDate	2025-04-23
AnnouncementXML	Submission_2025-04-23_00:31:42.412.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Core Facility
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-14 02:28:31	ID requested
⏵ 1	2025-04-23 00:31:43	announced

Samad M, Ulfenborg B, Soleimani Sani S, Bauz, á, Thorbr, ü, gge M, Mohan Shrestha M, Ohlsson C, Maliqueo M, Stener-Victorin E, Wernstedt Asterholm I, Benrick A, Maternal Adiponectin Decreases Placenta Nutrient Transport in Mice. FASEB J, 39(8):e70556(2025) [pubmed]

10.1096/fj.202403251rr;

submitter keyword: protein expression,high-fat diet, fetal liver, mouse, phosphopeptide

Anna Benrick
contact affiliation	Department of Physiology, Institute of Neuroscience and Physiology, University of Gothenburg, Sweden
contact email	anna.benrick@gu.se
lab head
Proteomics Core Facility
contact affiliation	SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact email	gupcf@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD025390

PRIDE project URI

• PRIDE
  1. PXD025390
     1. Label: PRIDE project
     2. Name: Maternal Adiponectin Decreases Placenta NutrientTransport in Mice