PXD025384

PXD025384 is an original dataset announced via ProteomeXchange.

Title	PfCDPK7 signaling regulates phospholipid biogenesis in Plasmodium falciparum
Description	Calcium Dependent Protein Kinases (CDPKs) play important function in calcium signaling at various stage of Plasmodium falciparum. CDPK7 is one such kinase, which plays a crucial role in the development of Plasmodium falciparum parasite. In order to understand the function of PfCDPK7, we performed comparative phosphoproteomic analysis of PfCDPK7 knockout mutant and wild-type lines. In brief, proteins from PfCDPK7 knockout mutant and wild-type parasites were isolated, digested with trypsin and labelled with iTRAQ tags. Phosphopeptides were then enriched from iTRAQ labeled peptides and subjected to LC-MS/MS analysis. This led to the identification of putative substrates of PfCDPK7 in the parasites. A comparative proteomic analysis was performed to measure the protein abundance changes in the PfCDPK7 mutant parasites.
HostingRepository	PRIDE
AnnounceDate	2021-11-08
AnnouncementXML	Submission_2021-11-08_03:30:12.015.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Plasmodium falciparum 3D7; NCBI TaxID: 36329;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-14 01:56:30	ID requested
⏵ 1	2021-11-08 03:30:12	announced

Dataset with its publication pending

submitter keyword: Orbitrap Fusion, iTRAQ, Plasmodium falciparum, CDPK7

T. S. Keshava Prasad
contact affiliation	Professor and Deputy Director, Center for Systems Biology and Molecular Medicine Yenepoya Research Centre Yenepoya (Deemed to be University) University Road, Deralakatte Mangalore 575018, India
contact email	keshav@yenepoya.edu.in
lab head
Keshava Prasad T. S.
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India.
contact email	keshav@ibioinformatics.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025384
     1. Label: PRIDE project
     2. Name: PfCDPK7 signaling regulates phospholipid biogenesis in Plasmodium falciparum