PXD025381
PXD025381 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphoproteomics analysis in mouse primary hepatocytes with recombinant GP73 or glucagon |
| Description | Isolated mouse primary hepatocytes were cultured at glucose- and phenol-free DMEM medium, supplemented with 10mM pyruvate sodium and 10mM sodium lactate, and the cells were incubated for 1hr with vehice, recombinant mGP73 (64nM), or glucagon (3μM) ,cells were washed twice with cold PBS and scraped with cold RIPA lysis buffer supplemented with proteases and phosphatases inhibitors. Phosphoproteomics assay was performed by Shanghai luming biological technology co., LTD (Shanghai, China). |
| HostingRepository | iProX |
| AnnounceDate | 2021-11-18 |
| AnnouncementXML | Submission_2021-11-17_19:04:27.598.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Luming Wan |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-04-13 21:24:55 | ID requested | |
| 1 | 2021-11-16 21:04:16 | announced | |
| ⏵ 2 | 2021-11-17 19:04:28 | announced | 2021-11-18: Update information. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: GP73, glucagon, mouse primary hepatocytes, phosphoproteomics |
Contact List
| Hui Zhong | |
|---|---|
| contact affiliation | Beijing Institute of Biotechnology |
| contact email | towall@yahoo.com |
| lab head | |
| Luming Wan | |
| contact affiliation | Beijing Institute of Biotechnology |
| contact email | wanluming9@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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