PXD025374

PXD025374 is an original dataset announced via ProteomeXchange.

Title	Culture and stimulation conditions influence protein expression of primary fibroblasts
Description	Tissue fibrosis is a common pathway to organ injury and failure. It is characterized by an excessive deposition of extracellular matrix (ECM) in organs. Deciphering the fibrogenic processes is of utmost importance, as there are few effective therapies in fibrotic diseases 1. Systemic sclerosis (SSc) is a prototypical disease where fibroblasts (Fb) are key effector cells as they differentiate into myofibroblasts in response to chronic inflammation under the influence of transforming growth factor beta 1 (TGF-β1) pathway 2–4. In this study, we compared the proteome of primary Fb in different culture and stimulation conditions. Primary dermal normal human Fb were cultured at passage P3, P5 and P7 with and without Fetal Bovine Serum (FBS). At fifth passage, Fb were stimulated or not with different concentrations of recombinant human active TGF-β1 (0.04, 1 and 5 ng/mL) during 24, 48 and 72 hours.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_07:56:56.909.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BRAY FABRICE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-13 04:49:26	ID requested
⏵ 1	2021-11-03 07:56:57	announced

Vivier S, Chepy A, Bray F, Guerrier T, Speca S, Flament S, Jendoubi M, Balden M, Rolando C, Hachulla E, Launay D, Dubucquoi S, Sobanski V, Passages in culture and stimulation conditions influence protein expression of primary fibroblasts. Proteomics, 22(3):e2100116(2022) [pubmed]

submitter keyword: fibrosis, mass spectrometry, primary fibroblasts, systemic sclerosis, TGF-β1

Fabrice Bray
contact affiliation	Univ. Lille, CNRS, USR 3290 - MSAP - Miniaturisation pour la Synthèse, l'Analyse et la Protéomique, F-59000 Lille, France
contact email	fabrice.bray@univ-lille.fr
lab head
BRAY FABRICE
contact affiliation	MSAP USR 3290
contact email	fabrice.bray@univ-lille.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025374
     1. Label: PRIDE project
     2. Name: Culture and stimulation conditions influence protein expression of primary fibroblasts