PXD025329

PXD025329 is an original dataset announced via ProteomeXchange.

Title	Monobromobimane labelling in early- and late-passage primary human mesemchymal stem cells subjected to 2 hour heat shock
Description	Monobromobimane (mBBr) labelling: mBBr labelling was carried out either immediately prior to 42°C, two hour heat shock, or immediately following heat shock. Medium was removed from culture flasks and cells were washed using PBS. Cells were then labelled by incubation at 37°C with 6 mL of 400 µM mBBr (Sigma-Aldrich, #B4380) for 10 minutes. Following labeling, 6 ml of 2 mM L-glutathione reduced (GSH, SigmaAldrich, #G4251) in PBS was added to quench the mBBr reaction. The quenched mBBr solution was removed and cells were washed with PBS. Mass spectrometry (MS) sample preparation, analysis and data processing: Six 1.6 mm steel beads (Next Advance Inc.) were added to the cell pellet tube with 30 µL SL-DOC (1.1% (w/w) sodium dodecyl sulfate (Sigma), 0.3% (w/w) sodium deoxycholate (Sigma), 25 mM ammonium bicarbonate (AB, Fluka), in de-ionised (DI) water containing 0.1% (v/v) protease inhibitor cocktail (Sigma), and 0.1% (v/v) phosphotase inhibitor cocktail (Sigma). Cells were homogen
HostingRepository	PRIDE
AnnounceDate	2021-06-15
AnnouncementXML	Submission_2021-06-15_04:55:53.323.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jack Llewellyn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; cysteine monobromobimane derivative; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-12 19:28:43	ID requested
⏵ 1	2021-06-15 04:55:53	announced

Dataset with its publication pending

submitter keyword: Human senescent heat shock MSC mesenchymal stem cell monobromobimane mBBr

Joe Swift
contact affiliation	The University of Manchester
contact email	joe.swift@manchester.ac.uk
lab head
Jack Llewellyn
contact affiliation	The University of Manchester
contact email	jdmllewellyn@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025329

PRIDE project URI

• PRIDE
  1. PXD025329
     1. Label: PRIDE project
     2. Name: Monobromobimane labelling in early- and late-passage primary human mesemchymal stem cells subjected to 2 hour heat shock