PXD025321 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrated molecular landscape perturbations underlie cellular responses during hyperammonemia |
Description | Ammonia is a cytotoxic molecule generated during cellular functions and by the gut microbiome. Dysregulated ammonia metabolism initiates a hyperammonemic stress response (HASR). Hyperammonemia occurs in many chronic diseases but there is limited understanding of the overall consequences of HASR. A comprehensive array of unbiased approaches was used to identify global responses during hyperammonemia with experimental validation of critical findings. Protein/gene expression and chromatin accessibility in hyperammonemic murine myotubes and mouse skeletal muscle tissue were analyzed by quantitative proteomics, RNA sequencing (RNAseq), and Assay for Transposase Accessible Chromatin Sequencing (ATACseq). Unique clusters of expression/accessibility changes were identified in hyperammonemic myotubes. In hyperammonemic mouse muscle and myotubes, we identified enrichment of pathways involved in oxidative phosphorylation, calcium signaling, hypoxia inducible factor-1, and apoptosis and regulation of protein synthesis were altered during HASR and were experimentally validated. Enrichment of senescence signaling and glycosylation pathways during HASR were novel observations. Comparisons with RNAseq data from skeletal muscle from human patients with cirrhosis revealed similar perturbations, demonstrating the translational relevance of our observations. We identified several novel regulatory pathways during HASR that are conserved in different models and across species, and whose dysregulation could significantly impact skeletal muscle structure and function. |
HostingRepository | PRIDE |
AnnounceDate | 2021-07-27 |
AnnouncementXML | Submission_2021-07-27_07:00:26.647.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ling Li |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-12 11:07:04 | ID requested | |
⏵ 1 | 2021-07-27 07:00:27 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: C2C12 myotubes, skeletal muscle, hyperammonemia |
Contact List
Srinivasan Dasarathy |
contact affiliation | Department of Gastroenterology, Hepatology & Nutrition, Cleveland Clinic |
contact email | DASARAS@ccf.org |
lab head | |
Ling Li |
contact affiliation | Cleveland Clinic |
contact email | lil5@ccf.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025321 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025321
- Label: PRIDE project
- Name: Integrated molecular landscape perturbations underlie cellular responses during hyperammonemia