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PXD025298

PXD025298 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleASB11 controls purinosome formation through PAICS ubiquitination
DescriptionPurine act as building block for DNA and RNA, provide cellular energy and signaling, and can generate cofactors such as NADH and coenzyme A. Purine de novo synthesis pathway begins with amino acids, ribose 5-phosphate, CO2 and NH3 with 6 enzymes and 10 enzymatic steps to convert PRPP to IMP. In previous study, under purine-depleted or other stimuli the six enzymes will form dynamic and reversible complex called purinosome to enhance the pathway flux. However, the mechanism of purinosome formation is unknown. Phosphoribosyl aminoimidazole succinocarboxamide synthetase (PAICS), an enzyme involved in the step7 and 8 of purine biosynthesis. In our lab, we used interactome and ubiquiylome to identify PAICS, which is a substrate of ASB11. ASB11 is a substrate adaptor of cullin 5 ubiquitin ligase complex and is able to bind PAICS for ubiquitination. Here, we found that ASB11 could increase purinosome formation without any stimuli. This effect depends on ASB11 E3 function, which can ubiquitinate PAICS on K74 site. We generate K74R mutation of PAICS so that ASB11 couldn’t ubiquitinate it. Interestingly, this mutant reduces the formation of purinosomes under purine-depleted and other stressed conditions. After further research, we found ASB11 expression level will increase under some specific conditions. Moreover, we analyzed TCGA database and found that the expression of ASB11 in melanoma cell is higher than normal cell. Consistent with our finding, melanoma cell has partially formed purinosome under basal condition because of the higher expression of ASB11. We guess that ASB11 may plays an important role in cancer cell.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:05:07.774.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMing-Chieh CHOU
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListisotope labeled residue
InstrumentOrbitrap Fusion; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-09 03:50:55ID requested
12023-10-06 05:49:38announced
22024-10-22 06:05:08announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: purine de novo synthesis, ASB11, PAICS, purinosome
Contact List
Ruey-Hwa Chen
contact affiliationIBC R602,128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
contact emailrhchen@gate.sinica.edu.tw
lab head
Ming-Chieh CHOU
contact affiliationAcademia Sinica
contact emailmcchou0517@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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