PXD025298

PXD025298 is an original dataset announced via ProteomeXchange.

Title	ASB11 controls purinosome formation through PAICS ubiquitination
Description	Purine act as building block for DNA and RNA, provide cellular energy and signaling, and can generate cofactors such as NADH and coenzyme A. Purine de novo synthesis pathway begins with amino acids, ribose 5-phosphate, CO2 and NH3 with 6 enzymes and 10 enzymatic steps to convert PRPP to IMP. In previous study, under purine-depleted or other stimuli the six enzymes will form dynamic and reversible complex called purinosome to enhance the pathway flux. However, the mechanism of purinosome formation is unknown. Phosphoribosyl aminoimidazole succinocarboxamide synthetase (PAICS), an enzyme involved in the step7 and 8 of purine biosynthesis. In our lab, we used interactome and ubiquiylome to identify PAICS, which is a substrate of ASB11. ASB11 is a substrate adaptor of cullin 5 ubiquitin ligase complex and is able to bind PAICS for ubiquitination. Here, we found that ASB11 could increase purinosome formation without any stimuli. This effect depends on ASB11 E3 function, which can ubiquitinate PAICS on K74 site. We generate K74R mutation of PAICS so that ASB11 couldn’t ubiquitinate it. Interestingly, this mutant reduces the formation of purinosomes under purine-depleted and other stressed conditions. After further research, we found ASB11 expression level will increase under some specific conditions. Moreover, we analyzed TCGA database and found that the expression of ASB11 in melanoma cell is higher than normal cell. Consistent with our finding, melanoma cell has partially formed purinosome under basal condition because of the higher expression of ASB11. We guess that ASB11 may plays an important role in cancer cell.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:05:07.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ming-Chieh CHOU
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	isotope labeled residue
Instrument	Orbitrap Fusion; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-09 03:50:55	ID requested
1	2023-10-06 05:49:38	announced
⏵ 2	2024-10-22 06:05:08	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: purine de novo synthesis, ASB11, PAICS, purinosome

Ruey-Hwa Chen
contact affiliation	IBC R602,128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan
contact email	rhchen@gate.sinica.edu.tw
lab head
Ming-Chieh CHOU
contact affiliation	Academia Sinica
contact email	mcchou0517@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025298
     1. Label: PRIDE project
     2. Name: ASB11 controls purinosome formation through PAICS ubiquitination