PXD025292

PXD025292 is an original dataset announced via ProteomeXchange.

Title	biotin RNA pulldown assay of lnc-GD2H and mass spectrometry
Description	Vectors carrying lnc-GD2H and antisense lnc-GD2H were linearized with the corresponding restriction enzymes to prepare template DNAs for in vitro transcription. Biotinylated RNAs were mixed with proteins extracted from C2C12 cells, followed by targeting of the RNAs with streptavidin beads. After SDS-PAGE following RNA pulldown, the differentially expressed proteins were enzymolyzed and used for MS analysis.
HostingRepository	PRIDE
AnnounceDate	2021-05-18
AnnouncementXML	Submission_2021-05-18_02:20:53.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Chen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-09 01:32:42	ID requested
⏵ 1	2021-05-18 02:20:54	announced

Dataset with its publication pending

submitter keyword: C2C12, myoblast, lnc-GD2H, mice, biotin RNA pulldown, MS

Rui Chen
contact affiliation	Guangdong Traditional Medical and Sports Injury Rehabilitation Research Institute,Guangdong Second Provincial General Hospital, Guangzhou, China
contact email	rui.c.med@163.com
lab head
Rui Chen
contact affiliation	Guangdong Second Provincial General Hospital
contact email	rui.c.med@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD025292

PRIDE project URI

• PRIDE
  1. PXD025292
     1. Label: PRIDE project
     2. Name: biotin RNA pulldown assay of lnc-GD2H and mass spectrometry