PXD025264

PXD025264 is an original dataset announced via ProteomeXchange.

Title	HyPro-MS analysis of proteins proximal to nuclear noncoding RNAs in HeLa cells
Description	The ability of RNAs to form specific contacts with other macromolecules provides an important mechanism for subcellular compartmentalization. We developed a suite of hybridization-proximity (HyPro) labeling technologies for unbiased discovery of proteins (HyPro-MS) and transcripts (HyPro-seq) associated with RNAs of interest in genetically unperturbed cells. To generate the HyPro-MS dataset reported here, fixed and permeabilized HeLa cells were hybridized with digoxigenin-labeled oligonucleotide probes against noncoding RNAs 45S, NEAT1 or PNCTR and proteins co-localizing with these RNAs were biotinylated in situ using a custom-engineered HyPro enzyme containing a digoxigenin-binding domain. Biotinylated proteins were then captured on streptavidin beads and analyzed by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2021-11-08
AnnouncementXML	Submission_2021-11-08_01:41:31.215.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025264
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eugene Makeyev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-08 06:01:05	ID requested
⏵ 1	2021-11-08 01:41:31	announced

Dataset with its publication pending

submitter keyword: Human, HeLa cells, LC-MS/MS, Orbitrap Fusion Lumos Tribrid

Eugene Makeyev
contact affiliation	Centre for Developmental Neurobiology, King’s College London, UK
contact email	eugene.makeyev@kcl.ac.uk
lab head
Eugene Makeyev
contact affiliation	King's College London
contact email	eugene.makeyev@kcl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025264

PRIDE project URI

• PRIDE
  1. PXD025264
     1. Label: PRIDE project
     2. Name: HyPro-MS analysis of proteins proximal to nuclear noncoding RNAs in HeLa cells