PXD025263

PXD025263 is an original dataset announced via ProteomeXchange.

Title	Unique Protein Interaction Networks Define The Chromatin Remodeling Module of The NuRD Complex
Description	The combination of four proteins and their paralogues including MBD2/3, GATAD2A/B, CDK2AP1, and CHD3/4/5, which we refer to as the MGCC module, form the chromatin remodeling module of the Nucleosome Remodeling and Deacetylase (NuRD) complex. To date, mechanisms by which the MGCC module acquires paralogue-specific function and specificity have not been addressed. Understanding the protein-protein interaction (PPI) network of the MGCC subunits is essential in defining underlying mechanisms of gene regulation. Therefore, using pulldown followed by mass spectrometry analysis (PD-MS) we report a proteome-wide interaction network of the MGCC module in a paralogue-specific manner. Our data also demonstrate that the disordered C-terminal region of CHD3/4/5 is a gateway to incorporate remodeling activity into both the ChAHP (CHD4, ADNP, HP1γ) and NuRD complexes in a mutually exclusive manner. We define a short aggregation prone region (APR) within the C-terminal segment of GATAD2B that is essential for the interaction of CHD4 and CDK2AP1 with the NuRD complex. Finally, we also report an association of CDK2AP1 with the Nuclear Receptor Co-Repressor (NCOR) complex. Overall, this study provides insight into the possible mechanisms through which the MGCC module can achieve specificity and diverse biological functions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:37:11.678.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	mehdi Sharifi tabar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-08 06:00:46	ID requested
1	2023-03-10 20:56:45	announced
⏵ 2	2023-11-14 08:37:13	announced	2023-11-14: Updated project metadata.

Sharifi Tabar M, Giardina C, Feng Y, Francis H, Moghaddas Sani H, Low JKK, Mackay JP, Bailey CG, Rasko JEJ, Unique protein interaction networks define the chromatin remodelling module of the NuRD complex. FEBS J, 289(1):199-214(2022) [pubmed]

submitter keyword: ChAHP complex, mass spectrometry, chromatin remodeling, NCOR complex, protein-protein interactions,NuRD complex, aggregation protein region

John Rasko
contact affiliation	PROFESSOR JOHN RASKO, AO BSc(Med), MBBS(Hons), PhD, MAICD, FFSc(RCPA), FRCPA, FRACP, FAHMS Head of Department, Cell & Molecular Therapies, Royal Prince Alfred Hospital, Professor, Faculty of Medicine & Health, The University of Sydney, Head, Gene and Stem Cell Therapy Program, Centenary Institute. Level 2, Building 89, Missenden Road, CAMPERDOWN NSW 2050, AUSTRALIA Tel 02 95656160 | Fax 02 9565 6101 | j.rasko@centenary.org.au‌
contact email	j.rasko@centenary.org.au
lab head
mehdi Sharifi tabar
contact affiliation	The centenary institute of cancer medicine
contact email	m.tabar@centenary.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD025263

PRIDE project URI

• PRIDE
  1. PXD025263
     1. Label: PRIDE project
     2. Name: Unique Protein Interaction Networks Define The Chromatin Remodeling Module of The NuRD Complex