PXD025255

PXD025255 is an original dataset announced via ProteomeXchange.

Title	Automated method for enrichment of newly synthesized proteins based on AHA labeling
Description	In this study, a novel high throughput method was successfully developed for the automated enrichment of newly synthesized proteins (NSPs) from complex environments, like cell culture media supplemented with fetal bovine serum (FBS). The proposed automated method allows for the robust and effective simultaneous enrichment of dozen of samples in a period of 3 hours. The enrichment method was comprehensively evaluated and validated, and was applied to the mass spectrometry (MS) analysis of the FBS-rich secretome of melanoma A375 cells treated with the immune cytokines interferon alpha (IFN-α) and gamma (IFN-γ).
HostingRepository	PRIDE
AnnounceDate	2023-05-14
AnnouncementXML	Submission_2023-05-14_14:20:06.596.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DavidVargas Diaz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-08 02:24:28	ID requested
1	2022-02-17 00:43:28	announced
⏵ 2	2023-05-14 14:20:07	announced	2023-05-14: Updated project metadata.

Vargas-Diaz D, Altelaar M, Automated High-Throughput Method for the Fast, Robust, and Reproducible Enrichment of Newly Synthesized Proteins. J Proteome Res, 21(1):189-199(2022) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: High throughput, newly synthesized proteins, AHA, LC-MS, interferon, melanoma, enrichment, Proteomics, Click chemistry

MaartenAltelaar
contact affiliation	Professor, Department of Pharmaceutical Sciences, Netherlaands Proteomics Centre, Utrecht University, The Netherlands
contact email	m.altelaar@uu.nl
lab head
DavidVargas Diaz
contact affiliation	Utrecht University
contact email	d.vargasdiaz@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025255

PRIDE project URI

• PRIDE
  1. PXD025255
     1. Label: PRIDE project
     2. Name: Automated method for enrichment of newly synthesized proteins based on AHA labeling