PXD025251

PXD025251 is an original dataset announced via ProteomeXchange.

Title	Transcriptomic points-of-departure (tPOD) derived from a 4-day rainbow trout embryo toxicity test were protective of apical effects for 17α-ethinylestradiol
Description	There is an urgent demand for more efficient and ethical approaches in chemical risk assessment. Using 17α-ethinylestradiol (EE2) as a model compound, this study established a live-animal alternative embryo benchmark-dose (BMD) assay for rainbow trout (RBT; Oncorhynchus mykiss) to derive transcriptomic points-of-departure (tPODs). Embryos were exposed to graded concentrations of EE2 (0, 1.13, 1.57, 6.22, 16.3, 55.1, and 169 ng/L) from hatch to 4 and up to 60 days post-hatch (dph) to assess molecular and apical responses, respectively. Whole proteome analyses of alevins did not show clear estrogenic effects, while transcriptomics revealed responses that were in agreement with apical effects including excessive accumulation of intravascular and hepatic proteinaceous fluid and significant increases in mortality at 55.1 and 169 ng/L EE2 at later time points. Transcriptomic BMD analysis estimated the median of the 20th lowest geneBMD to be 0.18 ng/L; the most sensitive tPOD. Other estimates (0.78, 3.64, and 1.63 ng/L for the tenth percentile geneBMD, first peak geneBMD distribution, and median geneBMD of the most sensitive overrepresented pathway, respectively) were within the same order of magnitude as empirically derived apical PODs for EE2 in the literature. This 4-day alternative RBT embryonic assay was effective in deriving protective tPODs for EE2.
HostingRepository	PRIDE
AnnounceDate	2021-08-17
AnnouncementXML	Submission_2021-08-17_12:22:22.347.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alper James Alcaraz
SpeciesList	scientific name: Oncorhynchus mykiss; NCBI TaxID: 8022;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-08 02:13:44	ID requested
⏵ 1	2021-08-17 12:22:23	announced

Alcaraz AJG, Mikul, á, š, ek K, Pot, ě, š, il D, Park B, Shekh K, Ewald J, Burbridge C, Zdr, á, hal Z, Schneider D, Xia J, Crump D, Basu N, Hecker M, -Ethinylestradiol in Rainbow Trout Using a 4-Day Transcriptomics Benchmark Dose (BMD) Embryo Assay. Environ Sci Technol, 55(15):10608-10618(2021) [pubmed]

submitter keyword: rainbow trout, transcriptomic point-of-departure, benchmark dose, ethinylestradiol, endocrine disruption, risk assessment

Markus Hecker
contact affiliation	Toxicology Center, University of Saskatchewan, Canada
contact email	markus.hecker@usask.ca
lab head
Alper James Alcaraz
contact affiliation	University of Saskatchewan
contact email	ajames.alcaraz@usask.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025251
     1. Label: PRIDE project
     2. Name: Transcriptomic points-of-departure (tPOD) derived from a 4-day rainbow trout embryo toxicity test were protective of apical effects for 17α-ethinylestradiol