PXD025239

PXD025239 is an original dataset announced via ProteomeXchange.

Title	Human, ovarian epithelial cancer cell line, LC-MSMS
Description	We investigated the functions of ECM1 subtypes ECM1a (inlcuding wild type and mutant) and ECM1b in ovarian cancer cell line HEYA8. While ECM1a is oncogenic, ECM1b is a tumor suppressor. To identify the molecules binding to ECM1a or ECM1b within cells, protein samples were generated by co-IP using HA antibody to pull down proteins potentially binding to ECM1a (HA-tagged) (samples: P20170700270A1/A2/A3/A4) or ECM1b (HA-tagged) (samples: P20170700270B1/B2/B3/B4) within cells indicated in gel image P20170700270. To identify more cell surface markers besides integrins alpha X and beta 2 binding to ECM1a-WT or ECM1a-MT, three batches of protein samples were generated by co-IP using HA antibody to pull down total proteins binding to ECM1a-WT or ECM1a-MT shown in gel image R20191201880, or to pull down membrane fractionation proteins binding to ECM1a-WT or ECM1a-MT indicated in gel image 20200100053, or to pull down membrane fractionation proteins of HEYA8 cells treated with ECM1a-WT or ECM1a-MT conditioned media binding to ECM1-WT or ECM1a-MT shown in gel image 20200100054.
HostingRepository	PRIDE
AnnounceDate	2021-05-27
AnnouncementXML	Submission_2021-07-20_01:56:20.560.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gong Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-07 03:09:14	ID requested
1	2021-05-27 00:03:49	announced
⏵ 2	2021-07-20 01:56:21	announced	2021-07-20: Updated publication reference for PubMed record(s): 34244494.

Yin H, Wang J, Li H, Yu Y, Wang X, Lu L, Lv C, Chang B, Jin W, Guo W, Ren C, Yang G, Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness. Nat Commun, 12(1):4230(2021) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Antibodies (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Human, Ovarian epithelial cell line, ECM1 subtypes, ECM1a wild type (WT), ECM1b, ECM1a mutant (MT)

GONG YANG
contact affiliation	Cancer Institute, Fudan University Shanghai Cancer Center
contact email	yanggong@fudan.edu.cn
lab head
Gong Yang
contact affiliation	Fudan University Shanghai Cancer Center
contact email	yanggong@fudan.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD025239

PRIDE project URI

• PRIDE
  1. PXD025239
     1. Label: PRIDE project
     2. Name: Human, ovarian epithelial cancer cell line, LC-MSMS