PXD025225

PXD025225 is an original dataset announced via ProteomeXchange.

Title	Temporal proteomics of p97/VCP inhibition
Description	To facilitate the development of p97 inhibitors as potential therapeutic agents and help define their clinical application, it is necessary to dissect the mechanism of action (MOA) of p97 inhibitors and compare them with proteasome inhibitors. Moreover, identifying specific cellular markers is critical for both the drug discovery and development process, to help validate candidate drugs and quantify their effect. In this study, we performed proteomic analysis and systematically compared p97 shRNA knockdown (KD), and three p97 inhibitors, with the proteasome inhibitor MG132 in HCT116 colon cancer cells. Through proteomic analyses we uncover the specific molecular mechanism by which p97 inhibition is distinct from proteasome inhibition, potentially relevant to understanding the efficacious effects of the former in solid tumors.
HostingRepository	PRIDE
AnnounceDate	2021-12-17
AnnouncementXML	Submission_2021-12-17_07:57:31.986.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025225
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Feng Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 10:40:34	ID requested
⏵ 1	2021-12-17 07:57:32	announced

Wang F, Li S, Houerbi N, Chou TF, Temporal proteomics reveal specific cell cycle oncoprotein downregulation by p97/VCP inhibition. Cell Chem Biol, 29(3):517-529.e5(2022) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: p97 inhibitor, proteasome inhibitor, HCT116, proteomic

Feng Wang
contact affiliation	BBE, Tsui-Fen Chou's lab, Caltech, USA
contact email	fengwang@caltech.edu
lab head
Feng Wang
contact affiliation	Caltech
contact email	fengwang@caltech.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025225
     1. Label: PRIDE project
     2. Name: Temporal proteomics of p97/VCP inhibition