PXD025202

PXD025202 is an original dataset announced via ProteomeXchange.

Title	Isolation of Arabidopsis EDS1 complexes from leaves with activated TNL RPS4/RRS1 signaling
Description	Nod-like receptors (NLRs) belong to AAA+ ATPases and act as ATP-dependent molecular switches, which detect activity of pathogens. Function of a large class of plant NLRs with Toll-like domain (TNL) is fully dependent on a class of EDS1-like proteins specific to seed plants. EDS1 (Enhanced Disease Susceptibility 1) – like proteins are defined as fusions of two domains: lipase-like a/b hydrolase domain and an a-helical bundle domain specific to the EDS1 family. In Arabidopsis, RRS1-RPS4 TNL signaling is dependent on the nuclear localization of EDS1 and formation of heterodimers between EDS1 and its sequence-related partners, PAD4 and SAG101. Here, we deposited results of affinity purification and LC-MS analyses are deposited for the EDS1 complexes after triggering NLR-dependent immune responses in Arabidopsis leaves. As a negative control, plants expressing GFP-tagged Telomere Repeat Binding 1 were used. The complexes were purified from nuclear enriched fractions of Arabidopsis complementation lines infected with the TNL-triggering bacteria.
HostingRepository	PRIDE
AnnounceDate	2021-04-06
AnnouncementXML	Submission_2021-07-19_23:08:15.535.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Christina Stolze
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 04:02:38	ID requested
1	2021-04-06 05:31:39	announced
⏵ 2	2021-07-19 23:08:16	announced	2021-07-20: Updated publication reference for PubMed record(s): 34099661.

Sun X, Lapin D, Feehan JM, Stolze SC, Kramer K, Dongus JA, Rzemieniewski J, Blanvillain-Baufum, é S, Harzen A, Bautor J, Derbyshire P, Menke FLH, Finkemeier I, Nakagami H, Jones JDG, Parker JE, Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity. Nat Commun, 12(1):3335(2021) [pubmed]

submitter keyword: TNL, PAD4, SAG101, helper NLR, immunity, Arabidopsis

Hirofumi Nakagami
contact affiliation	Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact email	nakagami@mpipz.mpg.de
lab head
Sara Christina Stolze
contact affiliation	Plant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne
contact email	stolze@mpipz.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD025202

PRIDE project URI

• PRIDE
  1. PXD025202
     1. Label: PRIDE project
     2. Name: Isolation of Arabidopsis EDS1 complexes from leaves with activated TNL RPS4/RRS1 signaling