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PXD025202

PXD025202 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIsolation of Arabidopsis EDS1 complexes from leaves with activated TNL RPS4/RRS1 signaling
DescriptionNod-like receptors (NLRs) belong to AAA+ ATPases and act as ATP-dependent molecular switches, which detect activity of pathogens. Function of a large class of plant NLRs with Toll-like domain (TNL) is fully dependent on a class of EDS1-like proteins specific to seed plants. EDS1 (Enhanced Disease Susceptibility 1) – like proteins are defined as fusions of two domains: lipase-like a/b hydrolase domain and an a-helical bundle domain specific to the EDS1 family. In Arabidopsis, RRS1-RPS4 TNL signaling is dependent on the nuclear localization of EDS1 and formation of heterodimers between EDS1 and its sequence-related partners, PAD4 and SAG101. Here, we deposited results of affinity purification and LC-MS analyses are deposited for the EDS1 complexes after triggering NLR-dependent immune responses in Arabidopsis leaves. As a negative control, plants expressing GFP-tagged Telomere Repeat Binding 1 were used. The complexes were purified from nuclear enriched fractions of Arabidopsis complementation lines infected with the TNL-triggering bacteria.
HostingRepositoryPRIDE
AnnounceDate2021-04-06
AnnouncementXMLSubmission_2021-07-19_23:08:15.535.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSara Christina Stolze
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-06 04:02:38ID requested
12021-04-06 05:31:39announced
22021-07-19 23:08:16announced2021-07-20: Updated publication reference for PubMed record(s): 34099661.
Publication List
Sun X, Lapin D, Feehan JM, Stolze SC, Kramer K, Dongus JA, Rzemieniewski J, Blanvillain-Baufum, é S, Harzen A, Bautor J, Derbyshire P, Menke FLH, Finkemeier I, Nakagami H, Jones JDG, Parker JE, Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity. Nat Commun, 12(1):3335(2021) [pubmed]
Keyword List
submitter keyword: TNL, PAD4, SAG101, helper NLR, immunity, Arabidopsis
Contact List
Hirofumi Nakagami
contact affiliationBasic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact emailnakagami@mpipz.mpg.de
lab head
Sara Christina Stolze
contact affiliationPlant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne
contact emailstolze@mpipz.mpg.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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