PXD025202 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isolation of Arabidopsis EDS1 complexes from leaves with activated TNL RPS4/RRS1 signaling |
Description | Nod-like receptors (NLRs) belong to AAA+ ATPases and act as ATP-dependent molecular switches, which detect activity of pathogens. Function of a large class of plant NLRs with Toll-like domain (TNL) is fully dependent on a class of EDS1-like proteins specific to seed plants. EDS1 (Enhanced Disease Susceptibility 1) – like proteins are defined as fusions of two domains: lipase-like a/b hydrolase domain and an a-helical bundle domain specific to the EDS1 family. In Arabidopsis, RRS1-RPS4 TNL signaling is dependent on the nuclear localization of EDS1 and formation of heterodimers between EDS1 and its sequence-related partners, PAD4 and SAG101. Here, we deposited results of affinity purification and LC-MS analyses are deposited for the EDS1 complexes after triggering NLR-dependent immune responses in Arabidopsis leaves. As a negative control, plants expressing GFP-tagged Telomere Repeat Binding 1 were used. The complexes were purified from nuclear enriched fractions of Arabidopsis complementation lines infected with the TNL-triggering bacteria. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-06 |
AnnouncementXML | Submission_2021-07-19_23:08:15.535.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sara Christina Stolze |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-06 04:02:38 | ID requested | |
1 | 2021-04-06 05:31:39 | announced | |
⏵ 2 | 2021-07-19 23:08:16 | announced | 2021-07-20: Updated publication reference for PubMed record(s): 34099661. |
Publication List
Sun X, Lapin D, Feehan JM, Stolze SC, Kramer K, Dongus JA, Rzemieniewski J, Blanvillain-Baufum, é S, Harzen A, Bautor J, Derbyshire P, Menke FLH, Finkemeier I, Nakagami H, Jones JDG, Parker JE, Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity. Nat Commun, 12(1):3335(2021) [pubmed] |
Keyword List
submitter keyword: TNL, PAD4, SAG101, helper NLR, immunity, Arabidopsis |
Contact List
Hirofumi Nakagami |
contact affiliation | Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany |
contact email | nakagami@mpipz.mpg.de |
lab head | |
Sara Christina Stolze |
contact affiliation | Plant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne |
contact email | stolze@mpipz.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025202
- Label: PRIDE project
- Name: Isolation of Arabidopsis EDS1 complexes from leaves with activated TNL RPS4/RRS1 signaling