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PXD025191

PXD025191 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes
DescriptionThe UVB component of the sunlight (290-320 nm) plays an important role in carcinogenesis through generation of high levels of bipyrimidine DNA photoproducts, while UVA (320-400 nm) has been associated with photoaging and tumor progression through generation of low, but continuous levels of DNA damage and oxidative stress. However, the contribution of UVA light to epidermal cell fate in the context of photoaging remains poorly understood. Here, by using proteomic analyses and biochemical assays for validation, we show that UVA induces proteome remodeling and senescence in primary keratinocytes, eliciting potent antioxidant and pro-inflammatory responses. As a model of early skin tumorigenesis during aging, immortalized non-malignant keratinocytes, bearing potentially oncogenic mutations and dysfunctional components of the senescent machinery , are resilient to UVA-induced stress, but are sensitive to paracrine oxidative stress and immune system activation induced by senescent neighboring keratinocytes. These observations reveal a new cellular mechanism by which UVA induces photoaging in the epidermis.
HostingRepositoryPRIDE
AnnounceDate2022-02-17
AnnouncementXMLSubmission_2022-02-17_00:33:15.966.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD025191
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHellen Valerio
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-06 04:01:38ID requested
12022-02-17 00:33:16announced
Publication List
Valerio HP, Ravagnani FG, Ronsein GE, Di Mascio P, A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes. Sci Rep, 11(1):23355(2021) [pubmed]
Keyword List
submitter keyword: NHEK, HaCaT, keratinocytes, UVA light, orbitrap fusion lumos, shotgun, senescence
Contact List
Paolo Di Mascio
contact affiliationDepartment of Biochemistry, Institute of Chemistry, Universidade de São Paulo, Brazil
contact emailpdmascio@usp.br
lab head
Hellen Valerio
contact affiliationUniversidade de São Paulo
contact emailhellen.valerio@usp.br
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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