PXD025191 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes |
Description | The UVB component of the sunlight (290-320 nm) plays an important role in carcinogenesis through generation of high levels of bipyrimidine DNA photoproducts, while UVA (320-400 nm) has been associated with photoaging and tumor progression through generation of low, but continuous levels of DNA damage and oxidative stress. However, the contribution of UVA light to epidermal cell fate in the context of photoaging remains poorly understood. Here, by using proteomic analyses and biochemical assays for validation, we show that UVA induces proteome remodeling and senescence in primary keratinocytes, eliciting potent antioxidant and pro-inflammatory responses. As a model of early skin tumorigenesis during aging, immortalized non-malignant keratinocytes, bearing potentially oncogenic mutations and dysfunctional components of the senescent machinery , are resilient to UVA-induced stress, but are sensitive to paracrine oxidative stress and immune system activation induced by senescent neighboring keratinocytes. These observations reveal a new cellular mechanism by which UVA induces photoaging in the epidermis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_00:33:15.966.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD025191 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Hellen Valerio |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-06 04:01:38 | ID requested | |
⏵ 1 | 2022-02-17 00:33:16 | announced | |
Publication List
Valerio HP, Ravagnani FG, Ronsein GE, Di Mascio P, A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes. Sci Rep, 11(1):23355(2021) [pubmed] |
Keyword List
submitter keyword: NHEK, HaCaT, keratinocytes, UVA light, orbitrap fusion lumos, shotgun, senescence |
Contact List
Paolo Di Mascio |
contact affiliation | Department of Biochemistry, Institute of Chemistry, Universidade de São Paulo, Brazil |
contact email | pdmascio@usp.br |
lab head | |
Hellen Valerio |
contact affiliation | Universidade de São Paulo |
contact email | hellen.valerio@usp.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025191 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025191
- Label: PRIDE project
- Name: A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes