PXD025191

PXD025191 is an original dataset announced via ProteomeXchange.

Title	A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes
Description	The UVB component of the sunlight (290-320 nm) plays an important role in carcinogenesis through generation of high levels of bipyrimidine DNA photoproducts, while UVA (320-400 nm) has been associated with photoaging and tumor progression through generation of low, but continuous levels of DNA damage and oxidative stress. However, the contribution of UVA light to epidermal cell fate in the context of photoaging remains poorly understood. Here, by using proteomic analyses and biochemical assays for validation, we show that UVA induces proteome remodeling and senescence in primary keratinocytes, eliciting potent antioxidant and pro-inflammatory responses. As a model of early skin tumorigenesis during aging, immortalized non-malignant keratinocytes, bearing potentially oncogenic mutations and dysfunctional components of the senescent machinery , are resilient to UVA-induced stress, but are sensitive to paracrine oxidative stress and immune system activation induced by senescent neighboring keratinocytes. These observations reveal a new cellular mechanism by which UVA induces photoaging in the epidermis.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_00:33:15.966.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025191
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hellen Valerio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 04:01:38	ID requested
⏵ 1	2022-02-17 00:33:16	announced

Valerio HP, Ravagnani FG, Ronsein GE, Di Mascio P, A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes. Sci Rep, 11(1):23355(2021) [pubmed]

submitter keyword: NHEK, HaCaT, keratinocytes, UVA light, orbitrap fusion lumos, shotgun, senescence

Paolo Di Mascio
contact affiliation	Department of Biochemistry, Institute of Chemistry, Universidade de São Paulo, Brazil
contact email	pdmascio@usp.br
lab head
Hellen Valerio
contact affiliation	Universidade de São Paulo
contact email	hellen.valerio@usp.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025191

PRIDE project URI

• PRIDE
  1. PXD025191
     1. Label: PRIDE project
     2. Name: A single dose of Ultraviolet-A induces proteome remodeling and senescence in primary human keratinocytes