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PXD025186

PXD025186 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe TDRD5/7-like protein, LOTR-1, functions within Z granules to balance epigenetic signals in the Caenorhabditis elegans germline
DescriptionLOTUS and Tudor domain containing proteins have critical roles in the germline. Proteins that contain these domains, such as Tejas/Tapas in Drosophila, help localize Vasa to the germ granules and facilitate piRNA transposon mediated silencing. The homologous proteins in mammals, TDRD5 and TDRD7, are required during spermiogenesis. Until now, LOTUS + Tudor domain proteins in C. elegans have remained elusive. Here we describe LOTR-1 (D1081.7), which derives its name from its LOTUS and Tudor domains. Interestingly, LOTR-1 docks next to P granules to colocalize with the Z-granule component ZNFX-1. LOTR-1’s Z-granule association requires its Tudor domain, but both LOTUS and Tudor deletions affect brood size when coupled with a knockdown of the Vasa homolog glh-1. LOTR-1 IP-mass spectrometry confirmed a Tudor-dependent association with Z-granule proteins ZNFX-1 and WAGO-1, but also with germ-granule proteins DEPS-1, the piRNA Argonaute PRG-1, and other WAGO-class Argonautes. Like znfx-1, lotr-1 mutants redistribute the coverage of 22G-RNAs toward the 5’ end of mutator targets and impact transgenerational epigenetic inheritance. Unlike znfx-1, the 5’ shift in 22G-RNA coverage does not extend to CSR-1 targets. Combined, these results suggest that LOTR-1 facilitates interactions between PRG-1/WAGO-class Argonautes, ZNFX-1 and target 3’UTRs to balance 22G-RNA distribution across mutator targets.
HostingRepositoryPRIDE
AnnounceDate2022-05-28
AnnouncementXMLSubmission_2022-05-27_22:49:47.685.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterF Butter
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-06 03:45:34ID requested
12022-05-27 22:49:48announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC-MS/MS
Contact List
Falk Butter
contact affiliationIMB, Ackermannweg 4, 55128 Mainz
contact emailf.butter@imb-mainz.de
lab head
F Butter
contact affiliationQuantitative Proteomics Institute of Molecular Biology (IMB)
contact emailf.butter@imb-mainz.de
dataset submitter
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Dataset FTP location
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