PXD025184

PXD025184 is an original dataset announced via ProteomeXchange.

Title	Glyoxal induces senescence of human keratinocytes through oxidative stress and activation of the AKT/FOXO3a/p27KIP1 pathway
Description	Senescence is a well-known cellular event characterized by specific markers like a permanent cell proliferation arrest and the secretion of messenger molecules forming the Senescence-Associated Secretory Phenotype (SASP). The SASP composition depends on many factors such as the cell type or the nature of the stress that induced senescence. Since the skin constitutes a barrier with the external environment, it is particularly subjected to different types of stresses and thus to premature cellular aging. The dicarbonyl compounds glyoxal and methylglyoxal are precursors of Advanced Glycation End-products (AGEs), known to be a feature of normal and pathological aging. In this study, we have demonstrated that glyoxal provokes oxidative stress by increasing ROS and AGEs levels and can induce senescence in human keratinocytes. An “early-stage” senescence phenotype characterized by a cell cycle arrest mediated by the AKT-FOXO3-p27KIP1 pathway and a “late-stage” senescence maintained by the p16INK4/pRb pathway were evidenced. Moreover, we characterized the resulting secretory phenotype during early senesecence by mass spectrometry. Our study provides evidence that glyoxal can affect keratinocyte function and act as a driver of human skin aging. Hence, senotherapeutics aimed at modulating glyoxal-associated senescence phenotype could be relevant to prevent the aging process in skin.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:15.486.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Salnot
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 03:18:23	ID requested
1	2022-01-03 02:43:03	announced
⏵ 2	2023-11-14 08:52:15	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: SASP, keratinocytes, advanced glycation end products,Cellular senescence, glyoxal, oxidative stress, dicarbonyl stress, secretory phenotype

Isabelle Petropoulos
contact affiliation	Sorbonne Université, CNRS, INSERM, Institut de Biologie Paris-Seine, Biological Adaptation and Ageing, B2A-IBPS, 75005 Paris, France
contact email	isabelle.petropoulos@sorbonne-universite.fr
lab head
Virginie Salnot
contact affiliation	3p5-proteom'ic plateform
contact email	virginie.salnot@parisdescartes.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD025184

PRIDE project URI

• PRIDE
  1. PXD025184
     1. Label: PRIDE project
     2. Name: Glyoxal induces senescence of human keratinocytes through oxidative stress and activation of the AKT/FOXO3a/p27KIP1 pathway