PXD025182

PXD025182 is an original dataset announced via ProteomeXchange.

Title	Drug target deconvolution in Plasmodium falciparum using thermal proteomics profiling
Description	Early identification of a compound’s mode of action can greatly benefit the discovery process. Thermal proteomics profiling (TPP) is a powerful, unbiased tool that can be used to identify potential ligands of protein targets. TPP takes advantage of the fact that a ligand binding to its target protein can significantly stabilise that protein, increasing its melting temperature (Tm). We previously optimised this technique for use in drug target deconvolution in the kinetoplastid parasite, Leishmania donovani1 and here report the optimisation and validation of TPP in the malaria-causing parasite P. falciparum.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:41:38.841.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victoriano Corpas-Lopez
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 03:17:36	ID requested
1	2022-10-13 06:25:27	announced
⏵ 2	2023-11-14 07:41:41	announced	2023-11-14: Updated project metadata.

Milne R, Wiedemar N, Corpas-Lopez V, Moynihan E, Wall RJ, Dawson A, Robinson DA, Shepherd SM, Smith RJ, Hallyburton I, Post JM, Dowers K, Torrie LS, Gilbert IH, Baraga, ñ, a B, Patterson S, Wyllie S, Lysyl tRNA Synthetase. ACS Infect Dis, 8(9):1962-1974(2022) [pubmed]

submitter keyword: lysine tRNA synthetase,thermal proteome profiling, plasmodium falciparum

Dr. Susan Wyllie
contact affiliation	Mode of Action Group, Biological Chemistry and Drug Discovery, School of Life Sciences, University of Dundee
contact email	swyllie@dundee.ac.uk
lab head
Victoriano Corpas-Lopez
contact affiliation	University of Dundee
contact email	vcorpaslopez@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD025182

PRIDE project URI

• PRIDE
  1. PXD025182
     1. Label: PRIDE project
     2. Name: Drug target deconvolution in Plasmodium falciparum using thermal proteomics profiling