PXD025175

PXD025175 is an original dataset announced via ProteomeXchange.

Title	LPC 18:1 protein interaction (OPC)
Description	Oligodendrocyte progenitor cells (OPC) were cultured in the presence of LPC 18:1-Cy5. Proteins interacting with LPC 18:1-Cy5 were UV crossed linked and cell lysates were separated via isoelectric focusing gel electrophoresis. Band was cut out from gel and processed for mass spectrometry. OPC lysates were also incubated with LPC 18:1-micelles and allowed to interact with OPC proteins. Samples were separated using sucrose gradient (Liposome floatation assay), top fraction was collected and processed for mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_00:13:59.963.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025175
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sanjoy Bhattacharya
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 00:44:41	ID requested
⏵ 1	2022-02-17 00:14:00	announced

Valdivia AO, Bhattacharya SK, Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function. eNeuro, 9(1):(2022) [pubmed]

submitter keyword: LPC 18:1, Protein-lipid complex, Liposome Floatation Assay, LPC 18:1-Cy5

Sanjoy Bhattacharya
contact affiliation	University of Miami Bascom Palmer Eye Institute
contact email	SBhattacharya@med.miami.edu
lab head
Sanjoy Bhattacharya
contact affiliation	Bascom Palmer Eye Institute, Department of Ophthalmology, Miller School of Medicine at University of Miami, Miami, FL 33136, USA
contact email	sbhattacharya@med.miami.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025175

PRIDE project URI

• PRIDE
  1. PXD025175
     1. Label: PRIDE project
     2. Name: LPC 18:1 protein interaction (OPC)