PXD025174

PXD025174 is an original dataset announced via ProteomeXchange.

Title	Proteome of long-term expanded human CD4+ and CD8+ T cells
Description	We analyzed the total proteome of CD4+ and CD8+ T cells isolated from human peripheral blood mononuclear cells (PBMC), and cultured to perform a CRISPR/CAS9 edition of their genome, in order to introduce an OST sequence at the C-terminus of proteins of interest (SLP76 or ZAP70, n=3 biological replicates in each case). Control T cells , isolated and cultured in the same way, but not modified by CRISPR/CAS9, were also analyzed (WT, n=3 or 6 biological replicates).
HostingRepository	PRIDE
AnnounceDate	2022-01-10
AnnouncementXML	Submission_2022-01-10_11:05:40.981.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anne Gonzalez de Peredo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-06 00:29:42	ID requested
⏵ 1	2022-01-10 11:05:41	announced

Dataset with its publication pending

submitter keyword: primary CD4+ and CD8+T cell, CRISPR/CAS9, whole cell lysate, proteome

Odile Schiltz
contact affiliation	Institute of Pharmacology and Structural Biology, CNRS, Toulouse, France
contact email	odile.schiltz@ipbs.fr
lab head
Anne Gonzalez de Peredo
contact affiliation	CNRS/IPBS
contact email	gonzalez@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD025174

PRIDE project URI

• PRIDE
  1. PXD025174
     1. Label: PRIDE project
     2. Name: Proteome of long-term expanded human CD4+ and CD8+ T cells