PXD025168

PXD025168 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Dynamic regulation of the proteome and lysine acetylome of Chlamydomonas reinhardtii in dependence on light and acetate
Description	Here, we performed a mass spectrometry-based analysis to study lysine acetylation and proteome dynamics in Chlamydomonas under varying growth conditions. Liquid cultures of Chlamydomonas were transferred from mixotrophic (light and acetate as carbon source) to heterotrophic (dark and acetate), or photoautotrophic (light only) growth conditions for 30 h before harvest. The proteome and acetylome changes between the different growth conditions were quantified using a stable isotope based dimethyl-labelling technique. The presence of lysine acetylation on nearly all enzymes involved in the glyoxylate cycle and its dynamic regulation in dependence on acetate was one of the major results of this study. Our results clearly show that lysine acetylation is dynamically regulated in Chlamydomonas in dependence on light and acetate. Furthermore, the newly identified lysine acetylation sites have a great potential for genetic engineering of metabolic pathways in Chlamydomonas.
HostingRepository	jPOST
AnnounceDate	2021-11-19
AnnouncementXML	Submission_2023-02-07_00:01:51.721.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jürgen Eirich
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; unknown modification; unknown modification; unknown modification; unknown modification; N6-acetyl-L-lysine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-05 23:44:40	ID requested
1	2021-11-19 02:28:49	announced
2	2022-09-18 03:42:50	announced	2022-09-18: Updated FTP location.
⏵ 3	2023-02-07 00:01:52	announced	2023-02-07: Updated PubMed.

Publication List

F, ü, ß, l M, K, ö, nig AC, Eirich J, Hartl M, Kleinknecht L, Bohne AV, Harzen A, Kramer K, Leister D, Nickelsen J, Finkemeier I, Dynamic light- and acetate-dependent regulation of the proteome and lysine acetylome of Chlamydomonas. Plant J, 109(1):261-277(2022) [pubmed]

F, ü, ß, l M, K, ö, nig AC, Eirich J, Hartl M, Kleinknecht L, Bohne AV, Harzen A, Kramer K, Leister D, Nickelsen J, Finkemeier I, Dynamic light- and acetate-dependent regulation of the proteome and lysine acetylome of Chlamydomonas. Plant J, 109(1):261-277(2022) [pubmed]

Keyword List

submitter keyword: chlamydomonas reinhardtii, PTM, Lysine acetylation, acK, DiMethyl

submitter keyword: chlamydomonas reinhardtii, PTM, Lysine acetylation, acK, DiMethyl

Contact List

Iris Finkemeier
lab head
Jürgen Eirich
contact affiliation	University of Münster - WWU - Institute for Biology and Biotechnology of Plants
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001115/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001115/