PXD025167 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Temporal proteomics of p97/VCP inhibition |
Description | To facilitate the development of p97 inhibitors as potential therapeutic agents and help define their clinical application, it is necessary to dissect the mechanism of action (MOA) of p97 inhibitors and compare them with proteasome inhibitors. Moreover, identifying specific cellular markers is critical for both the drug discovery and development process, to help validate candidate drugs and quantify their effect. In this study, we performed proteomic analysis and systematically compared p97 shRNA knockdown (KD), and three p97 inhibitors, with the proteasome inhibitor MG132 in HCT116 colon cancer cells. Through proteomic analyses we uncover the specific molecular mechanism by which p97 inhibition is distinct from proteasome inhibition, potentially relevant to understanding the efficacious effects of the former in solid tumors. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-15 |
AnnouncementXML | Submission_2021-12-15_08:08:18.776.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD025167 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Shan Li |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-05 23:42:29 | ID requested | |
⏵ 1 | 2021-12-15 08:08:19 | announced | |
Publication List
Wang F, Li S, Houerbi N, Chou TF, Temporal proteomics reveal specific cell cycle oncoprotein downregulation by p97/VCP inhibition. Cell Chem Biol, 29(3):517-529.e5(2022) [pubmed] |
Keyword List
ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
submitter keyword: p97 inhibitor, proteasome inhibitor, HCT116, proteomic |
Contact List
Shan Li |
contact affiliation | BBE, Tsui-Fen Chou's lab, Caltech, USA |
contact email | lflshan@caltech.edu |
lab head | |
Shan Li |
contact affiliation | Caltech |
contact email | lflshan@caltech.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD025167 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025167
- Label: PRIDE project
- Name: Temporal proteomics of p97/VCP inhibition