PXD025154

PXD025154 is an original dataset announced via ProteomeXchange.

Title	Differential exoproteome and biochemical characterisation of Neoparamoeba perurans
Description	Infection with the protozoan ectoparasite Neoparamoeba perurans, the causative agent of AGD, remains a global threat to salmonid farming. This study aimed to analyse the exoproteome of both an attenuated and virulent N. perurans isolate using proteomics and cytotoxicity testing. A disproportionate presence of proteins from the co-cultured microbiota of N. perurans was revealed on searching an amalgamated database of bacterial, N. perurans and Amoebozoa proteins. LC MS/MS identified 33 differentially expressed proteins, the majority of which were upregulated in the attenuated exoproteome. Proteins of interest found in both exoproteomes were maltoporin, ferrichrome-iron receptor, and putative enterobactin receptor. Protease activity remained significantly elevated in the attenuated exoproteome compared with the virulent exoproteome. Similarly, the attenuated exoproteome had a significantly higher cytotoxic effect on rainbow trout gill cell line (RTgill W1) cells compared with the virulent exoproteome. The presence of a phosphatase and serine protease in the virulent exoproteome may facilitate AGD infection but do not appear to be key players in causing cytotoxicity. Altogether this study reveals prolonged culture of N. perurans affects the exoproteome composition in favour of nutritional acquisition, and that the current culturing protocol for virulent N. perurans does not facilitate the secretion of virulence factors.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_00:13:02.519.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Orla Slattery
SpeciesList	scientific name: Paramoeba perurans; NCBI TaxID: 437603;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-01 08:19:17	ID requested
⏵ 1	2022-02-17 00:13:03	announced

N, í, Dhufaigh K, Botwright N, Dillon E, O'Connor I, MacCarthy E, Slattery O, . Microorganisms, 9(6):(2021) [pubmed]

submitter keyword: AGD, Neoparamaeba perurans, exoproteome

Orla Slattery
contact affiliation	Marine and Freshwater Research Centre, GMIT
contact email	orla.slattery@gmit.ie
lab head
Orla Slattery
contact affiliation	GMIT
contact email	orla.slattery@gmit.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025154
     1. Label: PRIDE project
     2. Name: Differential exoproteome and biochemical characterisation of Neoparamoeba perurans