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PXD025153

PXD025153 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTrypanosome ZFP3 arginine residues are subject to modification by multiple methyltransferases and essential for transcriptome regulation
DescriptionThe overall goal of this study was to identify methylated arginine residues on the Trypanosoma brucei RNA binding protein, ZFP3, and determine their functions in transcriptome regulation. We expressed Ty-tagged ZFP3 in procyclic form T. brucei, immunoprecipitated triplicate samples with anti-Ty antibodies, and subjected these samples to mass spectrometry to identify posttranslational modifications. We identified a highly methylated RGG domain, abundant R methylation at R109 in an unexpected context, and abundant tyrosine phosphorylation at Y4. To identify which protein arginine methyltransferases catalyzed these R methylation events, we performed in vitro methylation assays with TbPRMT1, TbPRMT6, and TbPRMT7 along with wild type and mutant ZFP3 substrates. These experiments showed that TbPRMT1 and TbPRMT7 methylate the RGG domain, while TbPRMT6 modifies R109. To ask how R methylations within affect ZFP3 transcriptome regulation, we overexpressed WT-ZFP3-Ty, R68,71K-ZFP3-Ty (hypomethylated) or R68,71F-ZFP3-Ty (methylmimic) in procyclic T. brucei. Overerexpression of WT-ZFP3-Ty lead to an increased expression of 749 tranascripts and decreased expression of 169 transcripts (1.5-fold change; padj<0.05). Overexpression of hypomethylated or methylmimic ZFP3 nearly abolished the capacity of ZFP3 to regulate three transcriptome, leading to changes in the abundances of less than 10 transcripts in either cell line. From these data, we conclude that arginine residues in the ZFP3 RGG domain are critical for its gene regulatory function.
HostingRepositoryPRIDE
AnnounceDate2021-05-26
AnnouncementXMLSubmission_2021-05-25_22:06:15.444.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShichen Shen
SpeciesList scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationListmethylated residue; dimethylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-01 08:19:17ID requested
12021-05-25 06:40:12announced
22021-05-25 22:06:15announced2021-05-26: Updated project metadata.
Publication List
10.1016/J.CHEMBIOL.2021.03.012;
Keyword List
submitter keyword: ZFP3, arginine methylation,Trypanosoma brucei, methyltransferases
Contact List
Laurie K. Read
contact affiliationDepartment of Microbiology and Immunology, Jacobs School of Medicine & Biomedical Sciences, University at Buffalo, Buffalo, NY 14203, USA
contact emaillread@buffalo.edu
lab head
Shichen Shen
contact affiliationUniversity at Buffalo
contact emailshichens@buffalo.edu
dataset submitter
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