PXD025152

PXD025152 is an original dataset announced via ProteomeXchange.

Title	Comprehensive proteomics for Discovery vaccine candidates against Streptococcus equi Infections
Description	Streptococcus equi subspecies equi (S. equi) is a major pathogen which cause strangles, a highly contagious respiratory infection, in horses and other equines.In this study, we discovered potential vaccine candidates using comprehensive proteomics and reverse vaccinology. As the initial step, we divided proteome of S. equi ATCC 39506 into whole cell lysate, secretory proteome, membrane proteome and extracellular vesicle and then, comparative proteomic analysis was performed to characterize the functional features of the proteome. Especially, extracellular vesicle of S. equi was evaluated at the first time. Total 114 potential vaccine candidates (PVCs) were selected using reverse vaccinology and knowledge based annotations. Comprehensive proteomic analysis confirmed that 60 PVCs were identified in S. equi ATCC 39506. Particularly, 32 PVCs were enriched in the EV proteome, suggesting that this cellular fraction may serve as vaccine.
HostingRepository	PRIDE
AnnounceDate	2021-08-06
AnnouncementXML	Submission_2021-08-06_02:08:10.842.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025152
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hayoung Lee
SpeciesList	scientific name: Streptococcus equi subsp. equi 4047; NCBI TaxID: 553482;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-01 07:09:27	ID requested
1	2021-08-05 17:32:14	announced
⏵ 2	2021-08-06 02:08:11	announced	2021-08-06: Updated project metadata.

Lee H, Yun SH, Hyon JY, Lee SY, Yi YS, Choi CW, Jun S, Park EC, Kim SI, Streptococcus equi-derived extracellular vesicles as a vaccine candidate against Streptococcus equi infection. Vet Microbiol, 259():109165(2021) [pubmed]

10.1016/J.VETMIC.2021.109165;

submitter keyword: Secretory proteome, Label-free, Membrane proteome,Streptococcus equi, Extracellular Vesicle, Whole cell lysate, LC-MS, comprehensive proteome

Seung Il Kim
contact affiliation	a Research Center for Bioconvergence Analysis, 458 Korea Basic Science Institute, Ochang 28119, Korea b Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea
contact email	ksi@kbsi.re.kr
lab head
Hayoung Lee
contact affiliation	Korea basic science institute
contact email	biryu32@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD025152

PRIDE project URI

• PRIDE
  1. PXD025152
     1. Label: PRIDE project
     2. Name: Comprehensive proteomics for Discovery vaccine candidates against Streptococcus equi Infections