PXD025151

PXD025151 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	An automated intact glycopeptide enrichment method for serum site-specific N-glycoproteomic analysis
Description	We developed an automated glycopeptide enrichment method for the analysis of serum site-specific N-glycoproteome. This automated method allowed for processing one sample within 20 min. It showed higher enrichment specificity, more intact glycopeptide identifications, and better quantitative reproducibility than the traditional manual method using micro-tip enrichment devices. We further applied this method to investigate the serum site-specific N-glycosylation changes between four patients with pancreatic cancer and seven healthy controls. The principal component analysis of intact N-glycopeptides showed a good clustering across cancer and normal groups. And we found that the site-specific glycoforms, mono-fucosylated and non-sialylated oligosaccharides, on IgG1 site 180 expressed a significant decrease in pancreatic cancer patients compared to healthy controls. Together, the automated method is a powerful tool for site-specific N-glycoproteomics analysis of complex biological samples, and it has great potential for clinical utilities.
HostingRepository	jPOST
AnnounceDate	2021-04-06
AnnouncementXML	Submission_2022-09-18_04:22:30.216.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luyao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-01 07:03:49	ID requested
1	2021-04-05 19:48:21	announced
2	2022-09-14 19:41:08	announced	2022-09-15: Updated PubMed.
⏵ 3	2022-09-18 04:22:31	announced	2022-09-18: Updated FTP location.

Publication List

Liu L, Zhu B, Fang Z, Zhang N, Qin H, Guo Z, Liang X, Yao Z, Ye M, Automated Intact Glycopeptide Enrichment Method Facilitating Highly Reproducible Analysis of Serum Site-Specific N-Glycoproteome. Anal Chem, 93(20):7473-7480(2021) [pubmed]

Liu L, Zhu B, Fang Z, Zhang N, Qin H, Guo Z, Liang X, Yao Z, Ye M, Automated Intact Glycopeptide Enrichment Method Facilitating Highly Reproducible Analysis of Serum Site-Specific N-Glycoproteome. Anal Chem, 93(20):7473-7480(2021) [pubmed]

Keyword List

submitter keyword: intact glycopeptide, serum N-glycoproteome

submitter keyword: intact glycopeptide, serum N-glycoproteome

Contact List

Mingliang Ye
lab head
Luyao
contact affiliation	CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS)
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001122/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001122/